Staining of normal human peripheral blood cells with Anti-Human HLA-ABC PE (left) and PE-Cy5 (right). Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.
Contents: Anti-Human HLA-ABC Functional Grade Purified Catalog Number: 16-9983 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, No NaN3 Storage Conditions: Store at 2-8°C. Handling Conditions: Use in sterile envrioment. Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay. Clone: W6/32 Host/Isotype: Mouse IgG2a, κ
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16-9983-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The W6/32 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I, HLA-A, B, C. MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells and are central in cell-mediated immune response and tumor surveillance. W6/32 mAb recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both 43 kDa and 11-12 kDa chains.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The W6/32 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The W6/32 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Barnstable, C. J., W. F. Bodmer, et al. 1978. Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis. Cell 14(1): 9-20.
Koene G, Arts-Hilkes, et al.2004. High level of aneuploidy of chromosome 6 by FISH analysis of head and neck squamous cell carcinoma: limited applicability of LOH analysis to define HLA loss. Hum Immunol. 65(12):1455-62. (IHC frozen, PubMed)
Goldman-Wohl D, Ariel I, et al. 2001. A study of human leukocyte antigen G expression in hydatidiform moles. Am J Obstet Gynecol. 185(2):476-80. (IHC frozen, PubMed)
Stern PL, Beresford N, et al. 1987. Class I-like MHC molecules expressed by baboon placental syncytiotrophoblast. J Immunol. 138(4):1088-91. (baboon cross-reactivity, PubMed)
