Product Information

Contents: Functional Grade Purified anti-human Toll-like receptor 2 (CD282, TLR2)
Catalog Number: 16-9922
Sizes: 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: TL2.1
Isotype: Mouse IgG2a, κ

Surface staining of normal human peripheral blood cells with anti-human Toll-like receptor 2 (TL2.1) PE. Appropriate isotype controls were used (open histogram). Cells in the monocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-9922	FITC anti-human Toll-like receptor 2 (CD282, TLR2, TLR-2)	488	518	FC
12-9922	PE anti-human Toll-like receptor 2 (CD282, TLR2, TLR-2)	488	575	FC
13-9922	Biotin anti-human Toll-like receptor 2 (CD282, TLR2, TLR-2)	N/A	N/A	FC
14-9922	Affinity Purified anti-human Toll-like receptor 2 (CD282, TLR2, TLR-2)	N/A	N/A	FC IHC IP WB
16-9922	Functional Grade* Purified anti-human Toll-like receptor 2 (CD282, TLR2, TLR-2)	N/A	N/A	FA FC
17-9922	Allophycocyanin (APC) anti-human Toll-like receptor 2 (CD282, TLR2, TLR-2)	633	660	FC
51-9922	Alexa Fluor® 647 anti-human Toll-like receptor 2 (CD282, TLR2, TLR-2)	633	668	FC
53-9922	Alexa Fluor® 488 anti-human Toll-like receptor 2 (CD282, TLR2, TLR-2)	488	519	FC
56-9922	Alexa Fluor® 700 anti-human Toll-like receptor 2 (CD282, TLR2, TLR-2) (APC-Cy5.5 Equivalent)	633	723	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The TL2.1 monoclonal antibody reacts with human Toll-like receptor 2 (TLR2). To date, at least ten members of the Toll family have been identified in human. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR2 is expressed by peripheral blood monocytes and is responsible for distinguishing different pathogens. TL2.1, a blocking antibody, has been used to study the role of TLR-2 as a pattern recognition receptor in microbial lipoprotein/lipopeptide induced cytokine production from human peripheral blood mononuclear cells. TL2.1 has been reported to immunoprecipitate human TLR2 (~90 kDa) from PBMC and HMEC.

Applications Reported

For research use only, not for diagnostic or therapeutic use. TL2.1 has been reported for use in flow cytometric analysis and blocking of TLR2-mediated cytokine production. Inhibition of cytokine production has been reported in the literature using Listeria monocytogenes and peptidoglycans. Optimal conditions for the inhibition assays using TL2.1 and specific stimulatory reagents/suppliers have to be evaluated by individual investigators.

Neutralization Protocol: (Important note: make sure to eliminate LPS contamination in the culture system)
1) Prepare target cells of interest at the concentration of 2x10⁶/ml in 10% FBS-IMDM (or 5% human serum).
2) Pre-incubate the cells with 10-20 μg/ml of TL2.1 or isotype control antibody at room temperature for 30min.
3) Add the stimuli and incubate at 37°C, 5% CO₂ for 14 hours. Harvest the supernatants.
4) Follow the protocol of eBioscience Ready-SET-Go ELISA set to detect IL-6 or TNF-α production.
For more detailed information, please visit http://www.ebioscience.com/ebioscience/appls/nutlr2.htm

Applications Tested

The TL2.1 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Cook EB, Stahl JL, Esnault S, Barney NP, Graziano FM. Toll-like receptor 2 expression on human conjunctival epithelial cells: a pathway for Staphylococcus aureus involvement in chronic ocular proinflammatory responses. Ann Allergy Asthma Immunol. 2005 Apr;94(4):486-97.(TL2.1 FA, PubMed)

Flo, T. H., O. Halaas, et al. (2000). "Human toll-like receptor 2 mediates monocyte activation by Listeria monocytogenes, but not by group B streptococci or lipopolysaccharide." J Immunol 164(4): 2064-9.

Lien, E., T. J. Sellati, et al. (1999). "Toll-like receptor 2 functions as a pattern recognition receptor for diverse bacterial products." J Biol Chem 274(47): 33419-25.

Faure, E., L. Thomas, et al. (2001). "Bacterial lipopolysaccharide and IFN-gamma induce Toll-like receptor 2 and Toll-like receptor 4 expression in human endothelial cells: role of NF-kappaB activation." J. Immunol 166(3): 2018-24.

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Pat. No. US 7,071,310