Product Information

Contents: Functional Grade Purified anti-human CD95 Ligand (Fas Ligand, CD178)
Catalog Number: 16-9919
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: NOK-1
Isotype: Mouse IgG1, κ
HLDA No.: N/A

Surface staining of human FasL tranfected cells with anti-human CD178 (NOK-1) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9919	Affinity Purified anti-human CD95 Ligand (Fas Ligand, CD178)	N/A	N/A	FA FC IP
16-9919	Functional Grade* Purified anti-human CD95 Ligand (Fas Ligand, CD178)	N/A	N/A	FA FC
13-9919	Biotin anti-human CD95 Ligand (Fas Ligand, CD178)	N/A	N/A	FC
12-9919	PE anti-human CD95 Ligand (Fas Ligand, CD178)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The NOK-1 monoclonal antibody reacts with human Fas (CD95) Ligand, a 40 kDa type II transmembrane glycoprotein. FasL is a member of the TNF family and is expressed by neutrophils, monocytes, and activated T cells and NK cells. The interaction of FasL with its receptor (CD95, Fas) induces Fas-mediated killing of lymphocytes. Human FasL is cleaved from the surface by matrix metalloproteinases (MMPs), resulting in a 26 kDa soluble form. Therefore for optimal detection of surface FasL on activated peripheral blood cells, incubation of cells with an MMP inhibitor is recommended.

Applications Reported

For research use only, not for diagnostic or therapeutic use. NOK-1 has been reported for use in flow cytometric analysis. NOK-1 has also been reported in blocking of FasL mediated killing in functional assays.

Applications Tested

The NOK-1 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Suda, T., H. Hashimoto, et al. (1997). "Membrane Fas ligand kills human peripheral blood T lymphocytes, and soluble Fas ligand blocks the killing." J Exp Med 186(12): 2045-50.
Kayagaki, N., A. Kawasaki, et al. (1995). "Metalloproteinase-mediated release of human Fas ligand." J Exp Med 182(6): 1777-83.
Tanaka, M., T. Suda, et al. (1995). "Expression of the functional soluble form of human fas ligand in activated lymphocytes." Embo J 14(6): 1129-35.

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