Product Information

Contents: Functional Grade Purified anti-human Toll-like receptor 1 (CD281, TLR1)
Catalog Number: 16-9911
Sizes: 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: GD2.F4
Isotype: Mouse IgG1, κ

Surface staining of normal human peripheral blood cells with anti-human Toll-like receptor 1 (GD2.F4) PE. Appropriate isotype controls were used (open histogram). Cells in the monocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9911	Affinity Purified anti-human Toll-like receptor 1 (CD281, TLR1, TLR-1)	N/A	N/A	FC IHC
16-9911	Functional Grade* Purified anti-human Toll-like receptor 1 (CD281, TLR1, TLR-1)	N/A	N/A	FC
12-9911	PE anti-human Toll-like receptor 1 (CD281, TLR1, TLR-1)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The GD2.F4 antibody reacts with human Toll-like receptor 1 (TLR1). TLR1 is expressed in low numbers on the surface of peripheral blood monocytes and even lower numbers on dendritic cells. A very high degree of donor variability has been reported for TLR1 expression. eBioscience manufactured GD2.F4 mAb has been tested by flow cytometry, in parallel with a sample from the developer using cells from healthy donors. eBioscience and the reference antibody gave similar results: donors tested confirmed low levels expression of TLR1 on PBMC (less than 1/4 log shift). While the ligand and exact function of TLR1 are not defined yet, it has been suggested that TLR1 may cooperate with TLR2 in response to certain antagonists. Despite low levels of surface expression as detected by flow cytometric analysis, TLRs are efficient signal transducing molecules in monocytes and dendritic cells. To date, at least ten members of the Toll family have been identified in human. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The GD2.F4 antibody has been reported for use in flow cytometric analysis. To date, inhibition of function has not been reported.

Applications Tested

The GD2.F4 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

A. Visintin, et al. "Regulation of toll-like receptors in human monocytes and dendritic cells." J. Immunol. 166: 249 (2001).
D.H. Wyllie, et al. "Evidence for an accessory protein function for toll-like receptor 1 in anti-bacterial responses." J. Immunol. 165: 7125 (2000).
Krutzik SR, Ochoa MT, Sieling PA, et al. Activation and regulation of Toll-like receptors 2 and 1 in human leprosy. Nat Med. 2003 May;9(5):525-532.

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