Product Information

Contents: Functional Grade Purified anti-human TACI (CD267)
Catalog Number: 16-9217
Sizes: 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENT STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: 11H3
Isotype: Mouse IgG2a

Staining of normal human peripheral blood cells with APC anti-human CD19 (HIB19) (cat. 17-0199) and .25 μg of Purified Mouse IgG2a, K Iso Cntrl (cat. 14-4724) (left) or .25 μg of Purified anti-human TACI (11H3) (right) followed by Biotin Anti-Mouse IgG (cat. 13-4013) and Streptavidin-PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9217	Affinity Purified anti-human TACI (CD267)	N/A	N/A	FA FC
16-9217	Functional Grade* Purified anti-human TACI (CD267)	N/A	N/A	FA FC
13-9217	Biotin anti-human TACI (CD267)	N/A	N/A	FC
12-9217	Phycoerythrin (PE) anti-human TACI (CD267)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 11H3 antibody reacts with human TACI, also known as tumor necrosis factor receptor 13B. TACI, like BCMA, binds BAFF and April. TACI and its ligands regulate humoral immune responses, activate NF-kB and c-jun N-terminal kinase, and are involved in B cell associated autoimmune diseases. TACI mRNA and protein are found predominantly in B-cells and correlate with BLyS binding in a panel of B-cell lines. TACI interacts with nanomolar affinity with the BLyS-related tumor necrosis factor homologue APRIL for which no clear in vivo role has been described. 11H3 does not block binding of BAFF/BLyS to TACI.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 11H3 antibody has been reported for use in flow cytometric analysis. 11H3 has also been reported to induce the expression of NF-κB in the human B cell line Namalwa.

Applications Tested

This 11H3 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

von Bulow GU, van Deursen JM, Bram RJ. Regulation of the T-independent humoral response by TACI. Immunity. 2001 May;14(5):573-82.

Ng LG, Sutherland AP, Newton R, Qian F, Cachero TG, Scott ML, Thompson JS, Wheway J, Chtanova T, Groom J, Sutton IJ, Xin C, Tangye SG, Kalled SL, Mackay F, Mackay CR. B cell-activating factor belonging to the TNF family (BAFF)-R is the principal BAFF receptor facilitating BAFF costimulation of circulating T and B cells. J Immunol. 2004 Jul 15;173(2):807-17.

Novak AJ, Darce JR, Arendt BK, Harder B, Henderson K, Kindsvogel W, Gross JA, Greipp PR, Jelinek DF. Expression of BCMA, TACI, and BAFF-R in multiple myeloma: a mechanism for growth and survival. Blood. 2004 Jan 15;103(2):689-94.

Sakurai D, Kanno Y, Hase H, Kojima H, Okumura K, Kobata T. TACI attenuates antibody production costimulated by BAFF-R and CD40. Eur J Immunol. 2007 Jan;37(1):110-8. PubMed

Sakurai D, Hase H, Kanno Y, Kojima H, Okumura K, Kobata T. TACI regulates IgA production by APRIL in collaboration with HSPG. Blood. 2007 Apr 1;109(7):2961-7. PubMed

Seshasayee D, Valdez P, Yan M, Dixit VM, Tumas D, Grewal IS. Loss of TACI causes fatal lymphoproliferation and autoimmunity, establishing TACI as an inhibitory BLyS receptor. Immunity. 2003 Feb;18(2):279-88.

Yan M, Wang H, Chan B, Roose-Girma M, Erickson S, Baker T, Tumas D, Grewal IS, Dixit VM. Activation and accumulation of B cells in TACI-deficient mice. Nat Immunol. 2001 Jul;2(7):638-43.

Related Products

Cat. 16-4724 Functional Grade Purified Mouse IgG2a, K Isotype Control
Cat. 14-6395 Anti-human TACI (CD267) polyclonal (clone Polyclonal)