Product Information

Contents: Functional Grade Purified anti-mouse Toll-like receptor 2 (CD282, TLR2)
Catalog Number: 16-9021
Sizes: 50 ug, 200 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: 6C2
Isotype: Rat IgG2b, κ

Staining of Raw264.7 cells with 0.5 μg FG Purified Rat IgG2b Iso Cntrl (cat. 16-4331) (open histogram) or 0.5 μg FG Purified anti-mouse TLR2 (6C2)(colored histogram) followed by Biotin Anti-Rat IgG (cat. 13-4813) and SAv-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9021	Affinity Purified anti-mouse Toll-like receptor 2 (CD282, TLR2, TLR-2)	N/A	N/A	FC IH/F IHC(Paraffin) IP
16-9021	Functional Grade* Purified anti-mouse Toll-like receptor 2 (CD282, TLR2, TLR-2)	N/A	N/A	FC
13-9021	Biotin anti-mouse Toll-like receptor 2 (CD282, TLR2, TLR-2)	N/A	N/A	FC IH/F
11-9021	FITC anti-mouse Toll-like receptor 2 (CD282, TLR2, TLR-2)	488	518	FC
12-9021	PE anti-mouse Toll-like receptor 2 (CD282, TLR2, TLR-2)	488	575	FC
17-9021	DISCONTINUED - Please see newly released Alexa Fluor® 647 6C2 as an alternate Allophycocyanin (APC) anti-mouse Toll-like receptor 2 (CD282, TLR2, TLR-2)	633	660	FC
51-9021	Alexa Fluor® 647 anti-mouse Toll-like receptor 2 (CD282, TLR2, TLR-2)	633	668	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 6C2 monoclonal antibody reacts with mouse Toll-like receptor 2 (TLR2). Mouse TLR2 is expressed by the myeloid lineage, including macrophage and dendritic cells in splenocytes and the RAW264.7 cell line. To date, at least twelve members of the Toll family have been identified in human and mouse. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR2 is expressed on the surface of cells and is responsible for distinguishing different pathogens.

Applications Reported

For research use only, not for diagnostic or therapeutic use. 6C2 has been reported for use in flow cytometric analysis. Unpublished preliminary studies indicate that this antibody may inhibit some aspects of monocyte activation through TLR2, however, it may not result in complete blocking of TLR2 function. For optimal flow cytometry staining with this antibody, it is recommended that a directly conjugated 6C2 (FITC, PE, or Biotin) be used in combination with FcR blocking with anti-mouse CD16/CD32 (clone 93, cat. 14-0161).

Applications Tested

The 6C2 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions or Raw264.7 cells. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Gibson FC 3rd, Hong C, Chou HH, Yumoto H, Chen J, Lien E, Wong J, Genco CA. 2004. Innate immune recognition of invasive bacteria accelerates atherosclerosis in apolipoprotein E-deficient mice. Circulation. 109(22):2801-6.

Paterson HM, Murphy TJ, Purcell EJ, Shelley O, Kriynovich SJ, Lien E, Mannick JA, Lederer JA. Injury Primes the Innate Immune System for Enhanced Toll-Like Receptor Reactivity. J Immunol. 2003 Aug 1;171(3):1473-1483.

Nilsen NJ, Nonstad U, Khan N, Knetter CF, Akira S, Sundan A, Espevik T, Lien E. 2004. Lipopolysaccharide and double-stranded RNA upregulate toll-like receptor 2 independently of myeloid differentiation factor 88. J Biol Chem. 279(38):39727-35.

Frasnelli ME, Tarussio D, et al. 2005. TLR2 modulates inflammation in zymosan-induced arthritis in mice. Arthritis Res Ther. 7(2):R370-9. (6C2, IHC frozen, PubMed)

Ziegler G, Harhausen D, et al. 2007. TLR2 has a detrimental role in mouse transient focal cerebral ischemia. Biochem Biophys Res Commun. 359(3):574-9. (6C2, IHC frozen, PubMed)

Hoffmann O, Braun JS, et al. 2007. TLR2 mediates neuroinflammation and neuronal damage. J Immunol. 15;178(10):6476-81. (6C2, ICC, PubMed)

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