Contents: Anti-Human CD338 (ABCG2) Functional Grade Purified Catalog Number: 16-8888 Concentration: 1 mg/ml Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, No NaN3 Storage Conditions: Store at 2-8°C. Handling Conditions: Use in sterile envrioment. Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay. Clone: 5D3 Host/Isotype: Mouse IgG2b, κ
Product Options
Cat. No.
Size
Price
Add Qty to Cart
16-8888-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The 5D3 monoclonal antibody reacts with the extracellular portion of the human ABCG2 protein, also known as Bcrp1 and MXR. The ABCG2 gene, a member of the multi-drug resistance (MDR) family, is highly expressed on primitive ‘side-population’ (SP) stem cells, which are defined by the efflux of fluorescent dyes such as Rhodamine 123 and Hoechest 33342. In the bone marrow, about 0.05% of cells display the low fluorescence and are highly enriched for repopulating cells. The SP cells, which express low or undetectable levels of CD34, have been identified in multiple species. In addition, expression of ABCG2 appears to be highly conserved.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 5D3 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The 5D3 antibody has been tested by flow cytometric analysis of human ABCG2 transfected cells and peripheral blood leukocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Kim M, Tunquist H et al. 2002. The multidrug resistance transporter ABCG2 (breast cancer resistance protein 1) effluxes Hoechst 33342 and is overexpressed in hematopoietic stem cells. Clin Cancer Res: 8(1):22-8. Scharenber CW, Harkey MA. et al. 2002. The ABCG2 transporter is an efficient Hoechst 33342 efflux pump and is preferentially expressed by immature human hemtopoietic progenitors. Blood 15;99(2):507-12. Zhou, S., J. D. Schuetz, et al. 2001. The ABC transporter Bcrp1/ABCG2 is expressed in a wide variety of stem cells and is a molecular determinant of the side-population phenotype. Nat Med 7(9): 1028-34. Goodell MA, Rosenzweig M, et al. 1997. Dye efflux studies suggest that hematopoietic stem cells expressing low or undetectable levels of CD34 antigen exist in multiple species. Nat Med 3(12):1337-45.
