Product Information

Contents: Functional Grade Purified anti-human GM-CSF
Catalog Number: 16-7336
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffered saline, pH 7.2, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENT STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: BVD2-23B6
Isotype: Rat IgG2a, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
16-7336	Functional Grade* Purified anti-human GM-CSF (Granulocyte/Macrophage-Colony Stimulating Factor, GMCSF)	N/A	N/A	ELISA cap NU
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

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Description

The BVD2-23B6 antibody reacts with human granulocyte/macrophage - colony stimulating factor (GM-CSF). The BVD2-23B6 antibody is a neutralizing antibody. Human GM-CSF is a 14 kDa factor produced mainly by activated T cells and macrophages. Other cell types, such as endothelium and fibroblasts, also secrete GM-CSF in response to TNF-α, IL-2, IL-1, and IFN-γ. GM-CSF stimulates growth of macrophages, granulocytes and dendritic cells. GM-CSF is found as a membrane-bound form and also as a complex associated with the extracellular matrix. Non-glycosylated GM-CSF is biologically active.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The BVD2-23B6 antibody has been reported for use in ELISA, ELISPOT, neutralization, and intracellular staining for flow cytometric analysis.

Applications Tested

The Functional Grade Purified BVD2-23B6 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of GM-CSF bioactivity.

The BVD2-23B6 antibody at 80-156ng /ml has been found to inhibit by 50% the biological effects of 100pg/ml human GM-CSF in a TF-1 cell proliferation assay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols section.

Related Products

Cat. 16-4321 Functional Grade Purified Rat IgG2a Isotype Control
Cat. 16-7331 Functional Grade Purified anti-mouse GM-CSF (Granulocyte/Macrophage-Colony Stimulating Factor, GMCSF) (clone MP1-22E9)