|
||||||||||||||||||
|
||||||||||||||||||
|
||||||||||||||||||||||||||||||||||||||||||||||||||||
Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq. DescriptionThe MP1-22E9 antibody reacts with mouse granulocyte/macrophage - colony stimulating factor (GM-CSF). The MP1-22E9 antibody is a neutralizing antibody. Mouse GM-CSF is a 14 kDa factor produced mainly by activated T cells and macrophages. Other cell types, such as endothelium and fibroblasts, also secrete GM-CSF in response to TNF-α, IL-2, IL-1, and IFN-γ. GM-CSF stimulates growth of macrophages, granulocytes and dendritic cells. GM-CSF is found as a membrane-bound form and also as a complex associated with the extracellular matrix. Non-glycosylated GM-CSF is biologically active. Applications ReportedFor research use only, not for diagnostic or therapeutic use. The MP1-22E9 antibody has been reported for use in ELISA, ELISPOT, neutralization, and intracellular staining for flow cytometric analysis. Applications TestedThe Functional Grade Purified MP1-22E9 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of GM-CSF bioactivity. The MP1-22E9 antibody at 1.25 ug/ml has been found to inhibit by 50% the biological effects of 1 ng/ml mouse GM-CSF in an MC/9 cell proliferation assay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols section. The MP1-22E9 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse GM-CSF (Granulocyte/Macrophage-Colony Stimulating Factor, GMCSF) in combination with the biotin MP1-31G6 (13-7332) antibody for detection and recombinant mouse GM-CSF (14-8331) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/ml - 8 pg/ml should be included in each ELISA plate. Related ProductsCat. 16-4321 Functional Grade Purified Rat IgG2a Isotype Control Cat. 13-7332 Biotin anti-mouse GM-CSF (Granulocyte/Macrophage-Colony Stimulating Factor, GMCSF) (clone MP1-31G6) Cat. 34-8041 Carrier-Free Recombinant Mouse IL-4 (Interleukin-4, IL4) Cat. 14-8331 Recombinant Mouse GM-CSF (Granulocyte/Macrophage-Colony Stimulating Factor, GMCSF) Cat. 34-8331 Carrier-Free Recombinant Mouse GM-CSF (Granulocyte/Macrophage-Colony Stimulating Factor, GMCSF) |
||||||||||||||||||||||||||||||||||||||||||||||||||||
![]() |
![]() |
|
Copyright © 2000-2009 eBioscience, Inc. For Research Use Only. Not for use in diagnostic procedures. Not for further distribution without written consent. |