Product Information

Contents: Functional Grade Purified anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)
Catalog Number: 16-7325
Sizes: 50 ug
Formulation: Phosphate buffer pH 7.2,
500 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENT STERILE.
Endotoxin Level: Less than 0.01 ng/ug antibody, as determined by the LAL assay.
Clone: 1F3F3D4
Isotype: Rat IgM, κ

Mouse splenocytes (500,000 cells per well) were activated with anti-CD3 for 48 hrs in mouse TNF-α ELISPOT assay. Control well is medium alone.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7325	Affinity Purified anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	N/A	N/A	ELISA cap ELISPOT
16-7325	Functional Grade* Purified anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	N/A	N/A	ELISPOT
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 1F3F3D4 antibody reacts with mouse tumor necrosis factor-alpha (TNF-α), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4⁺ T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 1F3F3D4 antibody has been reported for use in capture of mouse TNF-α by ELISA and ELISPOT.

Applications Tested

The 1F3F3D4 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse TNF-α (Tumor Necrosis Factor alpha) in combination with the biotinylated anti-mouse TNF-α XT3/XT22 monoclonal antibody cocktail (13-7326) for detection and recombinant mouse TNF-α (14-8321) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/ml - 15 pg/ml should be included in each ELISA plate.
Recognition of mouse TNF-α by this assay is not interfered with by 1000x excess soluble TNF-α receptor type I or II.
This mouse TNF-α assay does not cross react with rat TNF-α.

The functional grade purified 1F3F3D4 antibody has been tested by LAL assay to verify low endotoxin level and by ELISPOT assay in combination with the biotinylated anti-mouse TNF-α XT3/XT22 monoclonal antibody cocktail (13-7326) for detection. This is the recommended antibody pair for mouse TNF-α ELISPOT assay.

References

Lucas, R., K. Heirwegh, et al. (1990). "Generation and characterization of a neutralizing rat anti-rmTNF-alpha monoclonal antibody." Immunology 71(2): 218-23.

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