Product Information

Contents: Functional Grade Purified anti-mouse TNF-alpha (TNF-α)
Catalog Number: 16-7322
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: MP6-XT3
Isotype: Rat IgG1, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
16-7322	Functional Grade* Purified anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	N/A	N/A	NU
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The MP6-XT3 monoclonal antibody reacts with mouse Tumor Necrosis Factor-alpha (TNF-α), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4⁺ T cells. TNF-α has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF-α forms dimers and trimers and also exists as a 26 kDa membrane-bound form.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MP6-XT3 antibody has been reported for use in cytokine neutralization.

Applications Tested

The Functional Grade Purified MP6-XT3 antibody has been tested by LAL assay to verify low endotoxin level and has been tested for neutralization of TNF-α bioactivity.

The MP6-XT3 antibody at 0.60 ug/ml has been found to neutralize by 50% the biological effects of 1.0 ng/ml mouse TNF-α in an assay of TNF-α induced cytotoxicity of L929 cells. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols section.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Hunter, C. A., M. J. Litton, et al. (1994). "Immunocytochemical detection of cytokines in the lymph nodes and brains of mice resistant or susceptible to toxoplasmic encephalitis." J Infect Dis 170(4): 939-45.
Litton, M. J., B. Sander, et al. (1994). "Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B." J Immunol Methods 175(1): 47-58.
Abrams, J. S., M. G. Roncarolo, et al. (1992). "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." Immunol Rev 127: 5-24.

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