human IFNg antibody, Functional Grade Purified conjugate

Contents: Functional Grade Purified anti-human IFN-γ
Catalog Number: 16-7318
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: NIB42
Isotype: Mouse IgG1, κ

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16-7318-81

50 ug

16-7318-85

500 ug

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7318	Affinity Purified anti-human IFNg (Interferon-gamma, IFN-g)	N/A	N/A	ELISA cap
16-7318	Functional Grade* Purified anti-human IFNg (Interferon-gamma, IFN-g)	N/A	N/A	ELISA cap NU
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

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Description

The NIB42 antibody reacts with human interferon-gamma (IFN-γ). The NIB42 antibody is a neutralizing antibody. IFN-γ is an ~20 kDa factor produced by activated T, B and NK cells and is an anti-viral and anti-parasitic cytokine. IFN-γ in synergy with other cytokines, such as TNF-α, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-γ are exerted on a wide range of cell types expressing the high affinity receptors for IFN-γ. Glycosylation of IFN-γ does not affect its biological activity.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The NIB42 antibody has been reported for use in neutralization of human IFN-γ and for ELISA capture.

Applications Tested

The Functional Grade Purified NIB42 antibody has been tested by LAL assay to verify low endotoxin levels and has been tested for ELISA capture and in bioassay for neutralization of IFN-γ bioactivity. The NIB42 antibody at 5 ug/ml has been found to inhibit by 50% the biological effects of 1 ng/ml human IFN-γ(ND50), in an EMCV assay of A549 cell protection. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols section.

The NIB42 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human Interferon-gamma (IFN-g) in combination with the biotin 4S.B3 (13-7319) antibody for detection and recombinant human IFNg (39-8319) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 2-8 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/ml - 8 pg/ml should be included in each ELISA plate.
For ELISPOT capture, the alternative clone MD-1 is recommended.

References

Meager, A., S. Parti, et al. (1984). "Detection of hybridomas secreting monoclonal antibodies to human gamma interferon using a rapid screening technique and specificity of certain monoclonal antibodies to gamma interferon." J Interferon Res 4(4): 619-25.

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