Product Information

Contents: Functional Grade Purified anti-human Interferon-gamma (IFN-γ)
Catalog Number: 16-7317
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
\KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: MD-1
Isotype: Mouse IgG1, κ

Human PBMCs were activated with PMA/Ionomycin in human IFN-γ ELISPOT assay. Control well is medium alone.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7317	Affinity Purified anti-human IFNg (Interferon-gamma, IFN-g)	N/A	N/A	ELISA cap ELISPOT cap FA IC Flow IHC NU WB
16-7317	Functional Grade* Purified anti-human IFNg (Interferon-gamma, IFN-g)	N/A	N/A	ELISPOT cap
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The MD-1 antibody reacts with human, rhesus, cynomolgus and baboon interferon-gamma (IFN-γ), the signature Th1 cytokine, which is produced mainly by activated T cells, NK cells and B cells. IFN-γ is a dimeric protein. IFN-γ has antiviral and antiparasitic activities and also inhibits the proliferation of normal and transformed cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MD-1 antibody has been reported for use in intracellular staining and flow cytometric analysis, cytokine neutralization, immunohistochemical staining, ELISA Capture, and ELISPOT Capture. Fluorochrome conjugated 4S.B3 antibody is recommended for use in intracellular staining and flow cytometry. (Please use Functional Grade Purified MD-1 antibody, cat. 16-7317, in functional assays.)

Applications Tested

The MD-1 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human interferon-γ (IFN-γ) in combination with the biotinylated 4S.B3 (13-7319) antibody for detection and recombinant human IFN-γ (14-8319) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/ml - 8 pg/ml should be included in each ELISA plate.
The FG Purified format of the MD-1 antibody has been tested as the capture antibody in an ELISPOT assay (in conjunction with the biotinylated 4S.B3 antibody) and for neutralization of IFN-γ biological activity. A suitable range of concentrations of this antibody for ELISPOT capture is 1-4 µg/ml.
For in vitro neutralization, the MD-1 antibody at 30 ug/ml has been found to neutralize by 50% the effect of 1 ng/ml human IFN-γ. A more potent neutralizing antibody is clone NIB42.

References

Van der Meide, P.H., et al. 1985. J. Immuno. Meth. 79: 293.
Yazdanbakhsh, M., et al. 1993. Eur. J. Immuno. 23: 3312.
Besouw, N.M., et al. 1994. J. Med. Primatol. 23: 42.
Van der Meide, P.H., et al. 1995. J. Med. Primatol. 24: 271.
Hilkens, C. M. U., et al. 1996. J. Immunol. 156: 1722.

Related Products

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