Product Information

Contents: Functional Grade Purified anti-mouse Interferon-gamma (IFN-γ)
Catalog Number: 16-7313
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: AN-18
Isotype: Rat IgG1, κ

Splenocytes from mice pre-immunized with PLP139-151 peptide were recall activated 4 weeks later with PLP139-151 for 24 hours in mouse IFN-γ ELISPOT assay. Control well is medium alone.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7313	Affinity Purified anti-mouse IFNg (Interferon-gamma, IFN-g)	N/A	N/A	ELISA cap ELISPOT cap IP
16-7313	Functional Grade* Purified anti-mouse IFNg (Interferon-gamma, IFN-g)	N/A	N/A	ELISA ELISPOT
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

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Description

The AN-18 antibody reacts with mouse interferon-gamma (IFN-γ), a 15-20 kDa cytokine secreted by Th1 cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The AN-18 antibody has been reported for use in ELISA and ELISPOT assay. For neutralization of IFN-γ activity, the alternate clones R4-6A2 or XMG1.2 are recommended.

Applications Tested

The AN-18 antibody has been tested as the capture antibody in IFN-γ ELISPOT assay, in combination with the biotinylated R4-6A2 antibody for detection. A concentration of 1-4 ug/ml is recommended. For neutralization of IFN-γ activity, the alternate clones R4-6A2 or XMG1.2 are recommended.

References

Frasca D, Adorini L, Landolfo S, Doria G. 1988. Enhancement of suppressor T cell activity by injection of anti-IFN-gamma monoclonal antibody. J Immunol. 140: 4103-7.

Simon MM, Hochgeschwender U, Brugger U, Landolfo S. 1986. Monoclonal antibodies to interferon-gamma inhibit interleukin 2-dependent induction of growth and maturation in lectin/antigen-reactive cytolytic T lymphocyte precursors. J Immunol. 136: :2755-62.

Landolfo S, Cofano F, Gandino L, Gribaudo G, Prat M. 1985. Monoclonal antibodies against mouse gamma-interferon inhibit tumoricidal macrophage activation by T lymphocytes. Exp Cell Biol. 53: 265-9.

Prat M, Gribaudo G, Comoglio PM, Cavallo G, Landolfo S. 1984. Monoclonal antibodies against murine gamma interferon. Proc Natl Acad Sci U S A. 81: 4515-9.

Slade, S., et al. 1989. Production of interferon-gamma during infection of mice with Plasmodium chabaudi. Immunobiology. 179: 353.

Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology. A. Kruisbeek eds. Wiley-Interscience, New York. Unit 6.20.1.

Kort JJ, Kawamura K, et al. 2006. Efficient presentation of myelin oligodendrocyte glycoprotein peptides but not protein by astrocytes from HLA-DR2 and HLA-DR4 transgenic mice. J Neuroimmunol. 173(1-2):23-34. (ELISPOT, PubMed)

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