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Product Information


Contents: Functional Grade Purified anti-mouse Interferon-gamma (IFN-γ)
Catalog Number: 16-7313
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN3
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: AN-18
Isotype: Rat IgG1, κ
 
 
data image 1 data image 2
Splenocytes from mice pre-immunized with PLP139-151 peptide were recall activated 4 weeks later with PLP139-151 for 24 hours in mouse IFN-γ ELISPOT assay. Control well is medium alone. 

Available Formats of This Product
Cat. No. Format Excite
(nm)
Emit
(nm)
Reported Applications
14-7313 Affinity Purified anti-mouse IFNg (Interferon-gamma, IFN-g) N/A N/A ELISA cap  ELISPOT cap  IP 
16-7313 Functional Grade* Purified anti-mouse IFNg (Interferon-gamma, IFN-g) N/A N/A ELISA  ELISPOT 
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg  (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
  Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description


The AN-18 antibody reacts with mouse interferon-gamma (IFN-γ), a 15-20 kDa cytokine secreted by Th1 cells.


Applications Reported


For research use only, not for diagnostic or therapeutic use. The AN-18 antibody has been reported for use in ELISA and ELISPOT assay. For neutralization of IFN-γ activity, the alternate clones R4-6A2 or XMG1.2 are recommended.


Applications Tested


The AN-18 antibody has been tested as the capture antibody in IFN-γ ELISPOT assay, in combination with the biotinylated R4-6A2 antibody for detection. A concentration of 1-4 ug/ml is recommended. For neutralization of IFN-γ activity, the alternate clones R4-6A2 or XMG1.2 are recommended.


References


Frasca D, Adorini L, Landolfo S, Doria G. 1988. Enhancement of suppressor T cell activity by injection of anti-IFN-gamma monoclonal antibody. J Immunol. 140: 4103-7.
Simon MM, Hochgeschwender U, Brugger U, Landolfo S. 1986. Monoclonal antibodies to interferon-gamma inhibit interleukin 2-dependent induction of growth and maturation in lectin/antigen-reactive cytolytic T lymphocyte precursors. J Immunol. 136: :2755-62.
Landolfo S, Cofano F, Gandino L, Gribaudo G, Prat M. 1985. Monoclonal antibodies against mouse gamma-interferon inhibit tumoricidal macrophage activation by T lymphocytes. Exp Cell Biol. 53: 265-9.
Prat M, Gribaudo G, Comoglio PM, Cavallo G, Landolfo S. 1984. Monoclonal antibodies against murine gamma interferon. Proc Natl Acad Sci U S A. 81: 4515-9.
Slade, S., et al. (1989). Production of interferon-gamma during infection of mice with Plasmodium chabaudi. Immunobiology. 179: 353.
Abrams, J. (1995). Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology. A. Kruisbeek eds. Wiley-Interscience, New York. Unit 6.20.1.



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