Product Information

Contents: Functional Grade Purified anti-mouse Interferon-gamma (IFN-γ)
Catalog Number: 16-7312
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: R4-6A2
Isotype: Rat IgG1, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7312	Affinity Purified anti-mouse IFNg (Interferon-gamma, IFN-g)	N/A	N/A	NU
16-7312	Functional Grade* Purified anti-mouse IFNg (Interferon-gamma, IFN-g)	N/A	N/A	NU
13-7312	Biotin anti-mouse IFNg (Interferon-gamma, IFN-g)	N/A	N/A	ELISA det ELISPOT
36-7312	In Vivo Cytokine Capture Assay (IVCCA) In Vivo Capture Antibody / Functional Grade* Biotin anti-mouse IFNg (Interferon-gamma, IFN-g)	N/A	N/A	IVCCA
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The R4-6A2 antibody reacts with mouse interferon-γ (IFN-γ ), a homodimeric 15-20 kDa cytokine secreted by Th1 cells. The R4-6A2 antibody is a neutralizing antibody. IFN-γ is an ~20 kDa factor produced by activated T, B and NK cells and is an anti-viral and anti-parasitic cytokine. IFN-γ in synergy with other cytokines, such as TNF-α, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-γ are exerted on a wide range of cell types expressing the high affinity receptors for IFN-γ. Glycosylation of IFN-γ does not affect its biological activity.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The R4-6A2 antibody has been reported for use in cytokine neutralization.

Applications Tested

The Functional Grade Purified R4-6A2 antibody has been tested by LAL assay to verify low endotoxin levels and has been tested for ELISA capture and in bioassay for neutralization of IFN-γ bioactivity.

The R4-6A2 antibody at 15 ng/ml has been found to inhibit by 50% the biological effects of 1 ng/ml mouse IFN-γ (ND50), in an EMCV assay of L929 cell protection. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols section.

The R4-6A2 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse Interferon-gamma (IFN-g) in combination with the biotin XMG1.2 (13-7311) antibody for detection and recombinant mouse IFNg (14-8311) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/ml - 15 pg/ml should be included in each ELISA plate. Detailed instructions for Cytokine Sandwich ELISA Using Matched Antibody Pairs can be found in the BestProtocols section.

For ELISPOT capture, the Functional Grade Purified AN-18 antibody is recommended.

References

Spitalny, G. L. and E. A. Havell. 1984. Monoclonal antibody to murine gamma interferon inhibits lymphokine-induced antiviral and macrophage tumoricidal activities. J Exp Med. 159: 1560-5.
Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology. A. Kruisbeek eds. Wiley-Interscience, New York. Unit 6.20.1.
Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. 2003. The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology. Unit 6.28. J. Coligan, A. Kruisbeek, D. Margulies, E. Shevach, and W. Strober, eds. John Wiley and Sons, New York.
Finkelman, F.D., and S.C. Morris. 1999. Development of an assay to measure in vivo cytokine production in the mouse. Int. Immunology. 11: 1811-1818.

Related Products

Cat. 16-4301    Functional Grade Purified Rat IgG1 Isotype Control
Cat. 51-7172    Alexa Fluor® 647 anti-mouse IL-17A (Interleukin-17A, IL17A) (clone eBioTC11-18H10.1)
Cat. 53-7172    Alexa Fluor® 488 anti-mouse IL-17A (Interleukin-17A, IL17A) (clone eBioTC11-18H10.1)
Cat. 88-7174    Mouse IL-17A (Interleukin-17A, IL17A) ELISA Ready-SET-Go!
Cat. 88-7234    Mouse IL-23 (p19/p40, IL23) ELISA Ready-SET-Go!
Cat. 88-7874    Mouse IL-17A (Interleukin-17A, IL17A) ELISPOT Ready-Set-Go!
Cat. 14-8231    Recombinant Mouse IL-23 (Interleukin-23, IL23)