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Anti-Mouse IFN gamma Functional Grade Purified

Also known as: Interferon-gamma, IFN-g, IFNg

RUO: For Research Use Only
 
Contents: Anti-Mouse IFN gamma Functional Grade Purified
Catalog Number: 16-7311
Concentration: 1 mg/ml
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN3
Storage Conditions: Store at 2-8°C.
Handling Conditions: Use in sterile envrioment.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: XMG1.2
Host/Isotype: Rat IgG1, κ
 
Product Options
Cat. No. Size Price Add Qty to Cart
16-7311-81 50 ug
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16-7311-85 500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
 
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Available Formats
Cat. No.FormatExcite
(nm)
Emit
(nm)
Reported ApplicationsRegulatory Status
11-7311 Anti-Mouse IFN gamma FITC 488 518 IC Flow RUO
12-7311 Anti-Mouse IFN gamma PE 488 575 IC Flow RUO
13-7311 Anti-Mouse IFN gamma Biotin N/A N/A ELISA RUO
14-7311 Anti-Mouse IFN gamma Purified N/A N/A ELISA cap RUO
16-7311 Anti-Mouse IFN gamma Functional Grade* Purified N/A N/A NU RUO
17-7311 Anti-Mouse IFN gamma APC 633 660 IC Flow RUO
19-7311 Anti-Mouse IFN gamma Cy5 633 670 IC Flow RUO
25-7311 Anti-Mouse IFN gamma PE-Cy7 488 760 IC Flow RUO
45-7311 Anti-Mouse IFN gamma PerCP-Cy5.5 488 690 IC Flow RUO
48-7311 Anti-Mouse IFN gamma eFluor® 450 (Pacific Blue® replacement) 405 450 IC Flow RUO
51-7311 Anti-Mouse IFN gamma Alexa Fluor® 647 633 668 IC Flow RUO
53-7311 Anti-Mouse IFN gamma Alexa Fluor® 488 488 519 IC Flow RUO
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg  (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
  Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
 
Description
The XMG1.2 antibody reacts with mouse interferon-γ. The XMG1.2 antibody is a neutralizing antibody. Mouse IFN-γ is a 20 kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine. IFN-γ, in synergy with other cytokines such as TNF-α, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-γ are exerted on a wide range of cell types expressing the high affinity receptors for IFN-γ. Glycosylation of IFN-γ does not affect its biological activity.
Applications Reported
For research use only, not for diagnostic or therapeutic use.
Applications Tested
The Functional Grade Purified XMG1.2 antibody has been tested by LAL assay to verify low endotoxin levels and has been tested for ELISA capture and in bioassay for neutralization of IFN-γ bioactivity.

The XMG1.2 antibody at 15 ng/ml has been found to inhibit by 50% the biological effects of 1 ng/ml mouse IFN-γ (ND50), in an EMCV assay of L929 cell protection. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section.

The XMG1.2 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse Interferon-γ in combination with the biotin R4-6A2 (13-7312) antibody for detection and recombinant mouse IFN-γ (39-8311) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 0.25 – 1.0 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/ml - 15 pg/ml should be included in each ELISA plate. Detailed instructions for Cytokine Sandwich ELISA Using Matched Antibody Pairs can be found in the BestProtocols section.

For ELISPOT capture, the Functional Grade Purified AN-18 antibody is recommended.
References
Kimura A, Naka T, Kishimoto T. IL-6-dependent and -independent pathways in the development of interleukin 17-producing T helper cells. Proc Natl Acad Sci U S A. 2007 Jul 17;104(29):12099-104. (XMG1.2, IC flow, PubMed)

Zhang Y, Xu G, Zhang L, Roberts AI, Shi Y. Th17 cells undergo Fas-mediated activation-induced cell death independent of IFN-gamma. J Immunol. 2008 Jul 1;181(1):190-6. (XMG1.2, IC flow, PubMed)

Cho KS, Hill AB. T cell acquisition of APC membrane can impact interpretation of adoptive transfer experiments using CD45 congenic mouse strains. J Immunol Methods. 2008 Jan 31;330(1-2):137-45. (XMG1.2, IC flow, PubMed)

Feng X, Akiyoshi DE, Sheoran A, Singh I, Hanawalt J, Zhang Q, Widmer G, Tzipori S. Serial propagation of the microsporidian Enterocytozoon bieneusi of human origin in immunocompromised rodents. Infect Immun. 2006 Aug;74(8):4424-9. (XMG1.2, FA)

Hidalgo LG, Urmson J, Halloran PF. IFN-gamma decreases CTL generation by limiting IL-2 production: A feedback loop controlling effector cell production. Am J Transplant. 2005 Apr;5(4 Pt 1):651-61. (XMG1.2, NU, PubMed)

Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992 Jun;127:5-24.

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