mouse IFNg antibody, Functional Grade Purified conjugate

Contents: Functional Grade Purified anti-mouse IFN-γ
Catalog Number: 16-7311
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: XMG1.2
Isotype: Rat IgG1, κ

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16-7311-81

50 ug

16-7311-85

500 ug

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7311	Affinity Purified anti-mouse IFNg (Interferon-gamma, IFN-g)	N/A	N/A	ELISA cap
16-7311	Functional Grade* Purified anti-mouse IFNg (Interferon-gamma, IFN-g)	N/A	N/A	NU
13-7311	Biotin anti-mouse IFNg (Interferon-gamma, IFN-g)	N/A	N/A	ELISA
11-7311	FITC anti-mouse IFNg (Interferon-gamma, IFN-g)	488	518	IC Flow
12-7311	PE anti-mouse IFNg (Interferon-gamma, IFN-g)	488	575	IC Flow
17-7311	APC anti-mouse IFNg (Interferon-gamma, IFN-g)	633	660	IC Flow
19-7311	Cy5 anti-mouse IFNg (Interferon-gamma, IFN-g)	633	670	IC Flow
25-7311	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse IFNg (Interferon-gamma, IFN-g)	488	760	IC Flow
51-7311	Alexa Fluor® 647 anti-mouse IFNg (Interferon-gamma, IFN-g)	633	668	IC Flow
53-7311	Alexa Fluor® 488 anti-mouse IFNg (Interferon-gamma, IFN-g)	488	519	IC Flow
57-7311	Pacific Blue® anti-mouse IFNg (Interferon-gamma, IFN-g)	402	421	IC Flow
45-7311	PerCP-Cy5.5 anti-mouse IFNg (Interferon-gamma, IFN-g) (PE-Cy5.5 replacement)	488	690	IC Flow
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The XMG1.2 antibody reacts with mouse interferon-γ. The XMG1.2 antibody is a neutralizing antibody. Mouse IFN-γ is a 20 kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine. IFN-γ, in synergy with other cytokines such as TNF-α, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-γ are exerted on a wide range of cell types expressing the high affinity receptors for IFN-γ. Glycosylation of IFN-γ does not affect its biological activity.

Applications Reported

For research use only, not for diagnostic or therapeutic use.

Applications Tested

The Functional Grade Purified XMG1.2 antibody has been tested by LAL assay to verify low endotoxin levels and has been tested for ELISA capture and in bioassay for neutralization of IFN-γ bioactivity.

The XMG1.2 antibody at 15 ng/ml has been found to inhibit by 50% the biological effects of 1 ng/ml mouse IFN-γ (ND50), in an EMCV assay of L929 cell protection. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols section.

The XMG1.2 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse Interferon-γ in combination with the biotin R4-6A2 (13-7312) antibody for detection and recombinant mouse IFN-γ (39-8311) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 0.25 – 1.0 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/ml - 15 pg/ml should be included in each ELISA plate. Detailed instructions for Cytokine Sandwich ELISA Using Matched Antibody Pairs can be found in the BestProtocols section.

For ELISPOT capture, the Functional Grade Purified AN-18 antibody is recommended.

References

Kimura A, Naka T, Kishimoto T. IL-6-dependent and -independent pathways in the development of interleukin 17-producing T helper cells. Proc Natl Acad Sci U S A. 2007 Jul 17;104(29):12099-104. (XMG1.2, FC, PubMed)

Zhang Y, Xu G, Zhang L, Roberts AI, Shi Y. Th17 cells undergo Fas-mediated activation-induced cell death independent of IFN-gamma. J Immunol. 2008 Jul 1;181(1):190-6. (XMG1.2, FC, PubMed)

Cho KS, Hill AB. T cell acquisition of APC membrane can impact interpretation of adoptive transfer experiments using CD45 congenic mouse strains. J Immunol Methods. 2008 Jan 31;330(1-2):137-45. (XMG1.2, FC, PubMed)

Feng X, Akiyoshi DE, Sheoran A, Singh I, Hanawalt J, Zhang Q, Widmer G, Tzipori S. Serial propagation of the microsporidian Enterocytozoon bieneusi of human origin in immunocompromised rodents. Infect Immun. 2006 Aug;74(8):4424-9. (XMG1.2, FA)

Hidalgo LG, Urmson J, Halloran PF. IFN-gamma decreases CTL generation by limiting IL-2 production: A feedback loop controlling effector cell production. Am J Transplant. 2005 Apr;5(4 Pt 1):651-61. (XMG1.2, NU, PubMed)

Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992 Jun;127:5-24.

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