Contents: Anti-Mouse IFN gamma Functional Grade Purified Catalog Number: 16-7311 Concentration: 1 mg/ml Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, No NaN3 Storage Conditions: Store at 2-8°C. Handling Conditions: Use in sterile envrioment. Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay. Clone: XMG1.2 Host/Isotype: Rat IgG1, κ
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16-7311-81
50 ug
16-7311-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The XMG1.2 antibody reacts with mouse interferon-γ. The XMG1.2 antibody is a neutralizing antibody. Mouse IFN-γ is a 20 kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine. IFN-γ, in synergy with other cytokines such as TNF-α, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-γ are exerted on a wide range of cell types expressing the high affinity receptors for IFN-γ. Glycosylation of IFN-γ does not affect its biological activity.
Applications Reported
For research use only, not for diagnostic or therapeutic use.
Applications Tested
The Functional Grade Purified XMG1.2 antibody has been tested by LAL assay to verify low endotoxin levels and has been tested for ELISA capture and in bioassay for neutralization of IFN-γ bioactivity.
The XMG1.2 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse Interferon-γ in combination with the biotin R4-6A2 (13-7312) antibody for detection and recombinant mouse IFN-γ (39-8311) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 0.25 – 1.0 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/ml - 15 pg/ml should be included in each ELISA plate. Detailed instructions for Cytokine Sandwich ELISA Using Matched Antibody Pairs can be found in the BestProtocols section.
For ELISPOT capture, the Functional Grade Purified AN-18 antibody is recommended.
References
Kimura A, Naka T, Kishimoto T. IL-6-dependent and -independent pathways in the development of interleukin 17-producing T helper cells. Proc Natl Acad Sci U S A. 2007 Jul 17;104(29):12099-104. (XMG1.2, IC flow, PubMed)
Zhang Y, Xu G, Zhang L, Roberts AI, Shi Y. Th17 cells undergo Fas-mediated activation-induced cell death independent of IFN-gamma. J Immunol. 2008 Jul 1;181(1):190-6. (XMG1.2, IC flow, PubMed)
Cho KS, Hill AB. T cell acquisition of APC membrane can impact interpretation of adoptive transfer experiments using CD45 congenic mouse strains. J Immunol Methods. 2008 Jan 31;330(1-2):137-45. (XMG1.2, IC flow, PubMed)
Feng X, Akiyoshi DE, Sheoran A, Singh I, Hanawalt J, Zhang Q, Widmer G, Tzipori S. Serial propagation of the microsporidian Enterocytozoon bieneusi of human origin in immunocompromised rodents. Infect Immun. 2006 Aug;74(8):4424-9. (XMG1.2, FA)
