Product Information

Contents: Functional Grade Purified anti-mouse IL-17A (Interleukin-17A, IL17A)
Catalog Number: 16-7175
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffered saline, pH 7.2, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENT STERILE.
Clone: eBio17CK15A5
Isotype: Rat IgG2a, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7175	Affinity Purified anti-mouse IL-17A (Interleukin-17A, IL17A)	N/A	N/A	ELISA cap ELISPOT cap
16-7175	Functional Grade* Purified anti-mouse IL-17A (Interleukin-17A, IL17A)	N/A	N/A	ELISPOT cap
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

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Description

The eBio17CK15A5 antibody reacts with mouse IL-17A. Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse IL-17A receptor.

IL-23-dependent, IL-17A-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN-γ and IL-4, have each been found to negatively regulate the generation of these Th-17 cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The eBio17CK15A5 antibody has been reported useful for mouse IL-17A ELISA and ELISPOT capture.

Applications Tested

The Functional Grade Purified eBio17CK15A5 antibody has been tested by LAL assay to verify low endotoxin level and has been tested for mouse IL-17A ELISPOT and ELISA capture.

The Functional Grade Purified eBio17CK15A5 antibody has been tested as the capture antibody in a sandwich ELISA for measurement of mouse IL-17A protein levels, in combination with the biotinylated eBio17B7 antibody (13-7177) for detection and recombinant mouse IL-17A (14-8171) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1.0 – 4.0 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/ml - 15 pg/ml should be included in each ELISA plate.

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