Product Information

Contents: Functional Grade Purified anti-mouse IL-15 (Interleukin-15)
Catalog Number: 16-7154
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Clone: AIO.3
Isotype: Rat IgG2a, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
16-7154	Functional Grade* Purified anti-mouse IL-15 (Interleukin-15, IL15)	N/A	N/A	FA NU
36-7154	In Vivo Cytokine Capture Assay (IVCCA) In Vivo Capture Antibody / Functional Grade* Biotin anti-mouse IL-15 (Interleukin-15, IL15)	N/A	N/A	IVCCA
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

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Description

The AIO.3 antibody reacts with mouse IL-15. This is a neutralizing antibody. Mouse IL-15 is a 14-15 kDa factor, previously known as IL-T. IL-15 is produced by a variety of cells and has a role in the T cell-mediated immune response and the central nervous system. IL-15 binds to the β and γ subunits of the IL-2 receptor; however, these two cytokines have distinct biological activities. IL-15 is a specific maturation factor for NK cells and mimics the BM microenvironment for NK cell development in vitro. It is reported that IL-15 delivers an anti-apoptotic and survival signal for NK, T, and B cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The AIO.3 antibody is reported for use in neutralization of mouse IL-15 bioactivity.

Applications Tested

The Functional Grade Purified AIO.3 antibody has been tested by LAL assay to verify low endotoxin levels of < 0.01 ng/µg and in bioassay for neutralization of mouse IL-15 bioactivity.

The AIO.3 antibody at 0.39 ug/ml has been found to inhibit by 50% the biological effects of 50 ng/ml mouse IL-15 (14-8151) in a CTLL2 proliferation assay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols section.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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