Product Information

Contents: Functional Grade Purified anti-mouse IL-13 (Interleukin-13)
Catalog Number: 16-7135
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffered saline, pH 7.2, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENT STERILE.
Clone: eBio1316H
Isotype: Rat IgG1, κ

Mouse IL-13 ELISPOT: Splenocytes were activated with immobilized anti-CD3 and anti-CD28 antibodies for 24 hrs in a mouse IL-13 ELISPOT assay (right). Left well is medium alone control (no mitogen).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
16-7135	Functional Grade* Purified anti-mouse IL-13 (Interleukin-13, IL13)	N/A	N/A	NU
13-7135	Biotin anti-mouse IL-13 (Interleukin-13, IL13)	N/A	N/A	ELISA det ELISPOT det
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

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Description

The eBio1316H antibody reacts with mouse IL-13. The eBio1316H antibody is a neutralizing antibody. Mouse interleukin-13 (IL-13), originally designated p600, is a 11.6 kDa protein secreted by activated T helper cells, CD8+ T cells, and NK cells. IL-13 was originally identified in 1989 through differential hybridization of cDNA libraries prepared from T helper 1 and T helper 2 cells. IL-13 contains four cysteine residues, which form two intramolecular disulfide bonds. IL-13 shares many activities with IL-4; this relates to a requirement for IL-4Ralpha chain for signal transduction. The biological activities of IL-13 include suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression, and suppression of inflammatory cytokine expression. IL-13 induces CD23 expression on B cells and enhances CD72, and expression of class II MHC gene products. Mouse IL-13 does not display the activities on T cells and B cell stimulatory effects which IL-4 exhibits. Mouse IL-13 is active on human and mouse cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The eBio1316H antibody has been reported useful for neutralization, ELISA detection, and ELISPOT detection.

Applications Tested

The Functional Grade purified format of the eBio1316H antibody has been tested by LAL assay to verify low endotoxin level and by bioassay to quantitate neutralization of IL-13 biological activity.

For in vitro neutralization, the Functional Grade purified eBio1316H antibody at 0.04 ug/ml has been found to neutralize by 50% the biological effect of 7.5 ng/ml mouse IL-13 in an assay of TF-1 cell proliferation. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols section.

Related Products

Cat. 16-4301  Functional Grade Purified Rat IgG1 Isotype Control
Cat. 13-7133  Biotin anti-mouse IL-13 (Interleukin-13, IL13) (clone eBio13A)
Cat. 16-7139  Functional Grade Purified anti-human IL-13 (Interleukin-13, IL13) (clone PVM13-1)
Cat. 14-8131  Recombinant Mouse IL-13 (Interleukin-13, IL13)