mouse IL-12 antibody, Functional Grade Purified conjugate

Contents: Functional Grade Purified anti-mouse IL-12/IL-23 (p40 subunit)
Catalog Number: 16-7123
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: C17.8
Isotype: Rat IgG2a, κ

Product Options

Price

Cat. No.

Size

To view relevant pricing,
please select your country
and resume browsing.

16-7123-81

50 ug

16-7123-85

500 ug

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
16-7123	Functional Grade* Purified anti-mouse IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40	N/A	N/A	IC Flow IP NU WB
13-7123	Biotin anti-mouse IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40	N/A	N/A	ELISA det
12-7123	PE anti-mouse IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40	488	575	IC Flow
51-7123	Alexa Fluor® 647 anti-mouse IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23), p40	633	668	IC Flow
45-7123	PerCP-Cy5.5 anti-mouse IL-12 (Interleukin-12) / IL-23 (Interleukin-23) p40 (PE-Cy5.5 replacement)	488	690	IC Flow
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The C17.8 antibody reacts with the p40 subunit of IL-12 and IL-23. The C17.8 antibody has been found to neutralize IL-12 and IL-23 bioactivity. For specific neutralization of IL-23 only, please see clone G23-8 (anti-mouse IL-23 p19). IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted by activated monocytes, macrophages, and dendritic cells. IL-23 is a heterodimer composed of the disulfide-linked p19 protein and p40 protein, expressed by activated dendritic cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The C17.8 antibody has been reported for use in IP, Western, ELISA, neutralization, and intracellular staining for flow cytometric analysis.

Applications Tested

The Functional Grade Purified C17.8 antibody has been tested by LAL assay to verify low endotoxin levels and has been tested for ELISA detection and in bioassay for neutralization of IL-12 p70 and IL-23 bioactivity.

The C17.8 antibody at 62.0 ng/ml has been found to inhibit by 50% the biological effects of 1 ng/ml mouse IL-12 p70 (14-8121) or mouse IL-23 (14-8231), in corresponding bioassay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols section.

References

Cho D, Lee WJ, Halloran PJ, Trinchieri G, Kim YB.
Enhancement of porcine natural killer cell activity by recombinant human and murine IL-12. Cell Immunol. 1996 Aug 25;172(1):29-34.
Aragane Y, Riemann H, Bhardwaj RS, Schwarz A, Sawada Y, Yamada H, Luger TA, Kubin M, Trinchieri G, Schwarz T.
IL-12 is expressed and released by human keratinocytes and epidermoid carcinoma cell lines. J Immunol. 1994 Dec 15;153(12):5366-72.

Related Products

Cat. 16-4321  Functional Grade Purified Rat IgG2a Isotype Control
Cat. 14-7122  Affinity Purified anti-mouse IL-12 (Interleukin-12, IL12) (clone C18.2)
Cat. 88-7231  Mouse IL-23 (Interleukin-23, IL23) ELISA Ready-SET-Go! Kit (with Pre-Coated Plates)
Cat. 16-7232  Functional Grade Purified anti-mouse IL-23 (Interleukin-23, IL23) p19 (clone G23-8)
Cat. 88-7234  Mouse IL-23 (p19/p40, IL23) ELISA Ready-SET-Go!
Cat. 88-7921  Mouse IL-12 (Interleukin-12, IL12) p70 ELISA Ready-SET-Go! Kit (with Pre-Coated Plates)
Cat. 14-8231  Recombinant Mouse IL-23 (Interleukin-23, IL23)
Cat. 34-8231  Carrier-Free Recombinant Mouse IL-23 (Interleukin-23, IL23)