16-7115

Contents: Functional Grade Purified anti-human IL-1a (Interleukin-1 alpha)
Catalog Number: 16-7115
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: 28.9
Isotype: Mouse IgG1, κ

Description

The 28.9 antibody reacts with human interleukin-1α (IL-1α).

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 28.9 antibody has been reported for use as a detection antibody for human IL-1α ELISA and for neutralization of human IL-1α bioactivity.

Applications Tested

The biotinylated 28.9 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human IL-1α, in combination with the purified 364/3B3-14 (14-7118) antibody for capture and recombinant human IL-1α (14-8019) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/ml - 15 pg/ml should be included in each ELISA plate.
The Functional Grade purified 28.9 antibody has been tested for neutralization of human IL-1α bioactivity.

References

Munoz, C., et al. 1991. Dissociation between plasma and monocyte-associated cytokines during sepsis. Eur. J. Immunol. 21: 2177-2184.
Munoz, C., et al. 1991. Dysregulation of in vitro cytokine production by monocytes during sepsis. J. Clin. Invest. 88: 1747-1754.
Sassi, A., et al. 2005. Mechanisms of the natural reactivity of lymphocytes from noninfected individuals to membrane-associated leishmania infantum antigens. 174: 3598-3607. (ELISA)
Caricchio, R., et al. 2003. Ultraviolet B radiation-induced cell death: critical role of ultraviolet dose in inflammation and lupus autoantigen redistribution. J. Immunol. 171: 5778-5786. (Intracellular staining).
Garth, L.J., et al. 2001. Dendritic cell activation and cytokine production induced by group B neisseria meningitidis: interleukin-12 production depends on lipopolysaccharide expression in intact bacteria. Infect. Immunity. 69: 4351-4357. (Intracellular staining).

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