Contents: Functional Grade Purified anti-human interleukin-10 (IL-10)
Catalog Number: 16-7108
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN3
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: JES3-9D7
Isotype: Rat IgG1, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
|
|
Human peripheral blood mononuclear cells were stimulated with PMA and ionomycin in the presence of Brefeldin A for 5 hours. The cells were surface stained with FITC anti-human CD4 (RPA-T4) and intracellularly stained with PE anti-human IL-10 (JES3-9D7).
|
| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 12-7108 |
PE anti-human IL-10 (Interleukin-10, IL10) |
488 |
575 |
IC Flow
|
| 14-7108 |
Affinity Purified anti-human IL-10 (Interleukin-10, IL10) |
N/A |
N/A |
ELISA cap
ELISPOT cap
IHC(Paraffin)
|
| 16-7108 |
Functional Grade* Purified anti-human IL-10 (Interleukin-10, IL10) |
N/A |
N/A |
ELISPOT cap
NU
|
| 51-7108 |
Alexa Fluor® 647 anti-human IL-10 (Interleukin-10, IL10) |
633 |
668 |
IC Flow
|
| 53-7108 |
Alexa Fluor® 488 anti-human IL-10 (Interleukin-10, IL10) |
488 |
519 |
IC Flow
|
| 57-7108 |
Pacific Blue® anti-human IL-10 (Interleukin-10, IL10) |
402 |
421 |
IC Flow
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10).
Applications Reported
For research use only, not for diagnostic or therapeutic use. The JES3-9D7 antibody has been reported for use in ELISA capture, ELISPOT capture, neutralization, and intracellular staining for flow cytometric analysis.
Applications Tested
The JES3-9D7 antibody has been tested for neutralizing IL-10 binding and biological activity.
References
Abrams, J. S., M. G. Roncarolo, et al. 1992. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev 127: 5-24.
Chandler SW, Rassekh CH, et al. 2002. Immunohistochemical localization of interleukin-10 in human oral and pharyngeal carcinomas. Laryngoscope. 112(5):808-15. (IHC paraffin,
PubMed)
Sendide K, Deghmane AE, et al. 2005. Mycobacterium bovis BCG attenuates surface expression of mature class II molecules through IL-10-dependent inhibition of cathepsin S. J Immunol. 175(8):5324-32. (FA,
PubMed)
Related Products
Cat.
16-4301 Functional Grade Purified Rat IgG1 Isotype Control
Cat.
13-7109 Biotin anti-human IL-10 (Interleukin-10, IL10) (clone JES3-12G8)
