Contents: Functional Grade Purified anti-human interleukin-6 (IL-6)
Catalog Number: 16-7069
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN3
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: MQ2-13A5
Isotype: Rat IgG1, κ
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Human peripheral blood mononuclear cells were stimulated with LPS in the presence of Brefeldin A for 5 hours. The cells were intracellularly stained with anti-human IL-6 (MQ2-13A5) FITC (left) and with PE (right).
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-7069 |
Affinity Purified anti-human IL-6 (Interleukin-6, IL6) |
N/A |
N/A |
ELISA cap
ELISPOT cap
|
| 16-7069 |
Functional Grade* Purified anti-human IL-6 (Interleukin-6, IL6) |
N/A |
N/A |
NU
|
| 11-7069 |
FITC anti-human IL-6 (Interleukin-6, IL6) |
488 |
518 |
IC Flow
|
| 12-7069 |
PE anti-human IL-6 (Interleukin-6, IL6) |
488 |
575 |
IC Flow
|
| 56-7069 |
Alexa Fluor® 700 anti-human IL-6 (Interleukin-6) |
633 |
723 |
IC Flow
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The MQ2-13A5 antibody reacts with human interleukin-6 (IL-6), a 21-28 kDa cytokine secreted by a variety of cell types.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The MQ2-13A5 antibody has been reported for use in neutralizing IL-6 bioactivity, intracellular staining for flow cytometry, as well as ELISA.
Applications Tested
The MQ2-13A5 antibody has been tested for neutralizing IL-6 binding and biological activity
References
Andersson, J., J. Abrams, et al. Concomitant in vivo production of 19 different cytokines in human tonsils.1994. Immunology 83(1): 16-24.
Gaines Das, R. E. and S. Poole. The international standard for interleukin-6. Evaluation in an international collaborative study. 1993. J Immunol Methods 160(2): 147-53.
Related Products
Cat. 16-4301 Functional Grade Purified Rat IgG1 Isotype ControlCat. 13-7068 Biotin anti-human IL-6 (Interleukin-6, IL6) (clone MQ2-39C3)
