Product Information

Contents: Functional Grade Purified anti-mouse interleukin-6 (IL-6)
Catalog Number: 16-7061
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: MP5-20F3
Isotype: Rat IgG1, κ

Mouse splenocytes (500,000 cells per well) were activated with LPS (1 μg/ml) for 48 hours in mouse IL-6 ELISPOT assay. Control well is medium alone.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-7061	Fluorescein isothiocyanate (FITC) anti-mouse IL-6 (Interleukin-6, IL6)	488	518	IC Flow
12-7061	PE anti-mouse IL-6 (Interleukin-6, IL6)	488	575	IC Flow
14-7061	Affinity Purified anti-mouse IL-6 (Interleukin-6, IL6)	N/A	N/A	ELISA cap ELISPOT cap NU
16-7061	Functional Grade* Purified anti-mouse IL-6 (Interleukin-6, IL6)	N/A	N/A	ELISPOT cap NU
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The MP5-20F3 antibody reacts with mouse interleukin-6 (IL-6). The MP5-20F3 antibody is a neutralizing antibody. Mouse IL-6 (also known as B Cell Stimulating Factor, BCSF, and BSF-2) is a 21-28 kDa factor produced by a variety of cell types, including monocytes, fibroblasts and endothelial cells. Upon stimulation, macrophages, T, B, mast, and glial cells, eosinophils, keratinocytes and granulocytes also secrete IL-6. This pleiotrophic cytokine influences the antigen-specific immune response, inflammatory response, and the development of neurons, and is a major mediator of the acute phase reaction.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MP5-20F3 antibody has been reported for use in ELISA capture, ELISPOT capture, neutralization of IL-6 bioactivity, IHC, and intracellular staining for flow cytometric analysis.

Applications Tested

The Functional Grade Purified MP5-20F3 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of mouse IL-6 bioactivity.

The MP5-20F3 antibody at 0.03 ug/ml has been found to inhibit by 50% the biological effects of 0.01 ng/ml mouse IL-6 in an B9 cell proliferation assay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols section.

The MP5-20F3 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse interleukin-6 (IL-6) in combination with the biotinylated MP5-32C11 (13-7062) antibody for detection and recombinant mouse IL-6 (14-8061) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 0.5-2 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/ml - 8 pg/ml should be included in each ELISA plate.

References

Sander, B., I. Hoiden, et al. (1993). "Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining." J Immunol Methods 166(2): 201-14.
Starnes, H. F., Jr., M. K. Pearce, et al. (1990). "Anti-IL-6 monoclonal antibodies protect against lethal Escherichia coli infection and lethal tumor necrosis factor-alpha challenge in mice" J Immunol 145(12): 4185-91.

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