Contents: Functional Grade Purified anti-human interleukin-4 (IL-4)
Catalog Number: 16-7048
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN3
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: MP4-25D2
Isotype: Rat IgG1, κ
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Human peripheral blood mononuclear cells were stimulated with PMA and ionomycin in the presence of Brefeldin A for 5 hours. (Left) The cells were surface stained with PE anti-human CD3 (UCHT1) and intracellularly stained with FITC anti-human IL-4 (MP4-25D2). (Right) The cells were surface stained with FITC anti-human CD3 and intracellularly stained with PE anti-human IL-4.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-7048 |
Affinity Purified anti-human IL-4 (Interleukin-4, IL4) |
N/A |
N/A |
IC Flow
|
| 16-7048 |
Functional Grade* Purified anti-human IL-4 (Interleukin-4, IL4) |
N/A |
N/A |
NU
|
| 13-7048 |
Biotin anti-human IL-4 (Interleukin-4, IL4) |
N/A |
N/A |
ELISA det
ELISPOT det
|
| 11-7048 |
FITC anti-human IL-4 (Interleukin-4, IL4) |
488 |
518 |
IC Flow
|
| 12-7048 |
PE anti-human IL-4 (Interleukin-4, IL4) |
488 |
575 |
IC Flow
|
| 17-7048 |
APC anti-human IL-4 (Interleukin-4, IL4) |
633 |
660 |
IC Flow
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The MP4-25D2 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. MP4-25D2 has been reported for use in neutralizing IL-4 binding and biological activity. Please refer to the supporting protocols for more information. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Applications Tested
The MP4-25D2 antibody has been tested for neutralizing IL-4 binding and biological activity.
References
Ramanathan, L., R. Ingram, et al. (1993). Immunochemical mapping of domains in human interleukin 4 recognized by neutralizing monoclonal antibodies. Biochemistry 32(14): 3549-56.
Abrams, J. S., M. G. Roncarolo, et al. (1992). Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev 127: 5-24.
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