Contents: Functional Grade Purified anti-human interleukin-1β (IL-1β)
Catalog Number: 16-7018
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN3
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: CRM56
Isotype: Mouse IgG1
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
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*International customers: Please contact your distributor for region specific pricing.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 11-7018 |
Fluorescein (FITC) anti-human IL-1b (Interleukin-1 beta, IL1b) |
488 |
518 |
IC Flow
|
| 12-7018 |
Phycoerythrin (PE) anti-human IL-1b (Interleukin-1 beta, IL1b) |
488 |
575 |
IC Flow
|
| 14-7018 |
Affinity Purified anti-human IL-1b (Interleukin-1 beta, IL1b) |
N/A |
N/A |
ELISA cap
IC Flow
NU
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| 16-7018 |
Functional Grade* Purified anti-human IL-1b (Interleukin-1 beta, IL1b) |
N/A |
N/A |
NU
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*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The CRM56 antibody reacts with human and baboon interleukin-1β (IL-1β). IL-1β is a 17 kDa factor secreted primarily by monocytes. IL-1 has effects on T-helper cells, inducing IL-2 secretion and expression of IL-2 receptors. IL-1 has effects on B cells, promoting cell proliferation and immunoglobulin synthesis.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The CRM56 antibody has been reported for use in intracellular staining for flow cytometric analysis, cytokine neutralization, and ELISA. Fluorochrome conjugated CRM56 is recommended for use in intracellular staining for flow cytometry. Functional Grade purified CRM56 antibody, cat. 16-7018, is recommended for use in functional assays.
Applications Tested
The CRM56 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human interleukin-1ß (IL-1ß) in combination with the biotin CRM57 (13-7016) antibody for detection and recombinant human IL-1ß (14-8018) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1 - 4 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/ml - 8 pg/ml should be included in each ELISA plate.
The Functional Grade Purified CRM56 antibody has been tested by LAL assay to verify low endotoxin and by bioassay to verify neutralization of IL-1ß bioactivity.
For in vitro neutralization, the CRM56 antibody at 2.5 ug/ml has been found to neutralize by 50% the effect of 1 ng/ml human IL-1ß bioactivity in a D10.G4.1 cell proliferation bioassay.
Related Products
Cat.
16-4714 Functional Grade Purified Mouse IgG1, K Isotype Control
Cat.
13-7016 Biotin anti-human IL-1b (Interleukin-1 beta, IL1b) (clone CRM57)
Cat.
14-8018 Recombinant Human IL-1b (Interleukin-1 beta, IL1b)
