Product Information

Contents: Functional Grade Purified anti-mouse/rat interleukin-1β (IL-1β)
Catalog Number: 16-7012
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: B122
Isotype: Armenian Hamster IgG

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7012	Affinity Purified anti-mouse IL-1b (Interleukin-1 beta, IL1b)	N/A	N/A	ELISA cap NU
16-7012	Functional Grade* Purified anti-mouse IL-1b (Interleukin-1 beta, IL1b)	N/A	N/A	ELISA cap NU
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The B122 antibody reacts with mouse and rat interleukin-1β (IL-1β), a 17 kDa factor secreted primarily by monocytes. IL-1 has effects on T-helper cells, inducing IL-2 secretion and expression of IL-2 receptors.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The B122 antibody has been reported for use in cytokine neutralization and ELISA capture.

Applications Tested

The Functional Grade Purified B122 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of mouse IL-1β bioactivity.

The B122 antibody at 0.125 ug/ml has been found to inhibit by 50% the biological effects of 4.0 ng/ml mouse IL-1β in a D10 cell proliferation assay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols section.

The B122 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse interleukin-1 beta (IL-1β) in combination with the biotin anti-mouse Interleukin-1 beta (IL-1β) polyclonal (13-7112) antibody for detection and recombinant mouse IL-1β (14-8012) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/ml – 15 pg/ml should be included in each ELISA plate.

References

Hogquist, K, et al. 1991. Generation of monoclonal antibodies to murine IL-1beta and demonstration of IL-1 in vivo. J. Immunol. 146: 1534-1540.
Rogers, H.W., et al. 1992. Interleukin 1 participates in the development of anti-Listeria responses in normal and SCID mice. Proc Natl Acad Sci U S A. 89: 1011-5.

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