anti-mouse IL-1a (Interleukin-1 alpha, Interleukin-1a) antibody: Functional Grade

Contents: Functional Grade Purified anti-mouse interleukin-1α (IL-1α)
Catalog Number: 16-7011
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: ALF-161
Isotype: Armenian Hamster IgG

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
16-7011-81	50 ug	$90
16-7011-85	500 ug	$280

*International customers: Please contact your distributor for region specific pricing.

Mouse IL-1α ELISPOT Assay: Mouse peritoneal exudate cells were elicited with thioglycolate, harvested, and stimulated with 1 ug/ml LPS for 24 hrs. Control well is medium alone.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-7011	Fluorescein (FITC) anti-mouse IL-1a (Interleukin-1 alpha, IL1a)	488	518	IC Flow
12-7011	Phycoerythrin (PE) anti-mouse IL-1a (Interleukin-1 alpha, IL1a)	488	575	IC Flow
14-7011	Affinity Purified anti-mouse IL-1a (Interleukin-1 alpha, IL1a)	N/A	N/A	ELISA cap IC Flow NU
16-7011	Functional Grade* Purified anti-mouse IL-1a (Interleukin-1 alpha, IL1a)	N/A	N/A	NU
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The ALF-161 antibody reacts with mouse interleukin-1α (IL-1α). Mouse IL-1α, also called Lymphocyte Activating Factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), and Mononuclear Cell Factor (MCF), is a 17 kDa factor produced by a wide variety of cells, including macrophages, dendritic cells, T and B cells. IL-1α is mostly cell-associated, with 23% amino acid homology with IL-1β. The immune regulatory role of IL-1α is exerted on a wide range of cells including lymphocytes, epithelial cells and fibroblasts. In vivo, it induces hypotension, fever, and acute phase response.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The ALF-161 antibody has been reported for use in ELISA capture, ELISPOT capture, neutralization, and intracellular staining for flow cytometry.

Applications Tested

The Functional Grade Purified ALF-161 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of mouse IL-1α bioactivity.

The ALF-161 antibody at 0.031 ug/ml has been found to inhibit by 50% the biological effects of 4.0 ng/ml mouse IL-1α in a D10 cell proliferation assay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols section.

The ALF-161 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse interleukin-1 alpha (IL-1α) in combination with the biotin anti-mouse Interleukin-1 alpha (IL-1α) polyclonal (13-7111) antibody for detection and recombinant mouse IL-1α (14-8011) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000pg/ml - 8pg/ml should be included in each ELISA plate.

References

Fuhlbrigge, R., et al. 1988. Monoclonal antibodies to murine IL-1alpha. J. Immunol. 141: 2643-50.
Rogers, H.W., et al. 1992. Interleukin 1 participates in the development of anti-Listeria responses in normal and SCID mice. Proc Natl Acad Sci U S A. 89: 1011-5.

Related Products

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Cat. 13-7111    Biotin anti-mouse IL-1a (Interleukin-1 alpha, IL1a) polyclonal (clone Polyclonal)