Also known as: mucosal vascular addressin cell adhesion molecule , MAdCAM1
RUO: For Research Use Only
Surface staining of mouse TNFα stimulated bEnd.3 cell line with Anti-Mouse MAdCAM-1 FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
Contents: Anti-Mouse MAdCAM-1 Functional Grade Purified Catalog Number: 16-5997 Concentration: 1 mg/ml Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, No NaN3 Storage Conditions: Store at 2-8°C. Handling Conditions: Use in sterile envrioment. Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay. Clone: MECA-367 Host/Isotype: Rat IgG2a, κ
Product Options
Cat. No.
Size
Price
Add Qty to Cart
16-5997-81
50 ug
16-5997-82
100 ug
16-5997-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The MECA-367 monoclonal antibody reacts with mouse mucosal vascular addressin-1 (MAdCAM-1), a 50-60 kDa member of the Ig superfamily. This antigen is expressed in mucosal lymphoid tissue and lamina propria and is a counter-receptor for CD62L and CD49d. MAdCAM-1 is involved in homing of subpopulation of memory T cells to the gut lamina propria, and recirculation of naďve lymphocytes in Peyer’s patches and mesenteric lymph nodes.
Applications Reported
For research use only, not for diagnostic or therapeutic use. MECA-367 can be used in flow cytometric analysis, immunohistochemical staining of frozen mouse tissue sections, blocking in adhesion assays, immunoprecipitation and immunoblotting (WB).
Applications Tested
The MECA-367 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocyte suspensions and can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in other applications.
References
Streeter, P. R., B. T. Rouse, et al. (1988). Immunohistologic and functional characterization of a vascular addressin involved in lymphocyte homing into peripheral lymph nodes." J Cell Biol 107(5): 1853-62.
Streeter, P. R., E. L. Berg, et al. (1988). "A tissue-specific endothelial cell molecule involved in lymphocyte homing." Nature 331(6151): 41-6.
Transplantation of embryonic spleen tissue reveals a role for adult non-lymphoid cells in initiating lymphoid tissue organization.
Glanville SH, Bekiaris V, Jenkinson EJ, Lane PJ, Anderson G, Withers DR.
Eur J Immunol. 2009 Jan;39(1):280-9. (MECA-367, IF, PubMed)
The role of lymphoid tissue inducer cells in splenic white pulp development.
Withers DR, Kim MY, Bekiaris V, Rossi SW, Jenkinson WE, Gaspal F, McConnell F, Caamano JH, Anderson G, Lane PJ.
Eur J Immunol. 2007 Nov;37(11):3240-5. (MECA-367, IF, PubMed)
Functional involvement of P-selectin and MAdCAM-1 in the recruitment of alpha4beta7-integrin-expressing monocyte-like cells to the pregnant mouse uterus.
Fernekorn U, Butcher EC, Behrends J, Hartz S, Kruse A.
Eur J Immunol. 2004 Dec;34(12):3423-33. (MECA-367, blocking, PubMed)
Mouse syngenic in vitro blood-brain barrier model: a new tool to examine inflammatory events in cerebral endothelium.
Coisne C, Dehouck L, Faveeuw C, Delplace Y, Miller F, Landry C, Morissette C, Fenart L, Cecchelli R, Tremblay P, Dehouck B.
Lab Invest. 2005 Jun;85(6):734-46. (MECA-367, IF, PubMed)
Mucosal vascular addressin cell adhesion molecule-1 is expressed outside the endothelial lineage on fibroblasts and melanoma cells.
Leung E, Kanwar RK, Kanwar JR, Krissansen GW.
Immunol Cell Biol. 2003 Aug;81(4):320-7. (MECA-367, IF, PubMed)
