mouse B7RP-1 antibody, Functional Grade Purified conjugate

Contents: Functional Grade Purified anti-mouse B7RP-1 (CD275, B7-RP1, B7RP1, ICOS-L, ICOSL, B7h, GL50, B7-H2, B7H2)
Catalog Number: 16-5985
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: HK5.3
Isotype: Rat IgG2a, κ

Product Options		Price
Cat. No.	Size	To view relevant pricing, please select your country and resume browsing.
16-5985-81	50 ug
16-5985-82	100 ug
16-5985-85	500 ug

Two-color surface staining of mouse splenocytes with anti-mouse B7RP-1 (HK5.3) PE and anti-mouse CD3e (145-2C11) FITC. Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5985	Affinity Purified anti-mouse B7RP-1 (CD275, B7-RP1, B7RP1, ICOS-L, ICOSL, B7h, GL50, B7-H2, B7H2)	N/A	N/A	FA FC
16-5985	Functional Grade* Purified anti-mouse B7RP-1 (CD275, B7-RP1, B7RP1, ICOS-L, ICOSL, B7h, GL50, B7-H2, B7H2)	N/A	N/A	FA FC
13-5985	Biotin anti-mouse B7RP-1 (CD275, B7-RP1, B7RP1, ICOS-L, ICOSL, B7h, GL50, B7-H2, B7H2)	N/A	N/A	FC
12-5985	PE anti-mouse B7RP-1 (CD275, B7-RP1, B7RP1, ICOS-L, ICOSL, B7h, GL50, B7-H2, B7H2)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The HK5.3 monoclonal antibody reacts with mouse B7RP-1, also known as B7h, B7-H2, GL50 and ICOS Ligand. B7RP-1, a member of the B7 family, has a predicted molecular weight of ~40 kDa and belongs to the Ig superfamily. Mouse B7RP-1 is expressed by antigen presenting cells including B cells, monocytes/macrophages and dendritic cells. B7RP-1 binds to the ICOS molecule (AILIM, CRP-1) expressed by activated T cells. The interaction of ICOS/B7RP-1 plays an important role in the T cell costimulation pathway.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HK5.3 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking in functional assays.

Applications Tested

The HK5.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Iwai H, Kozono Y, et al. 2002. Amelioration of collagen-induced arthritis by blockade of inducible costimulator-b7 homologous protein costimulation. J Immunol. 169(8): 4332-9. Carreno BM, Collins M. 2002. THE B7 FAMILY OF LIGANDS AND ITS RECEPTORS: New Pathways for Costimulation and Inhibition of Immune Responses. Annu Rev Immunol. 20: 29-53. Liu X, Bai XF, et al. 2001. B7H costimulates clonal expansion of, and cognate destruction of tumor cells by, CD8(+) T lymphocytes in vivo. J Exp Med. 194(9): 1339-48.

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