Anti-Mouse CD274 (B7-H1) Functional Grade Purified

Also known as: B7H1, PD-L1, PDL-1, PDL1

RUO: For Research Use Only

mouse B7-H1 antibody, Functional Grade Purified conjugate

Staining of mouse splenocytes with Anti-Mouse CD274 (B7-H1) PE Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Contents: Anti-Mouse CD274 (B7-H1) Functional Grade Purified
Catalog Number: 16-5982
Concentration: 1 mg/ml
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 2-8°C.
Handling Conditions: Use in sterile envrioment.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: MIH5
Host/Isotype: Rat IgG2a, λ

Product Options
Cat. No.	Size	Price	Add Qty to Cart
16-5982-81	50 ug	To view relevant pricing, please select your country and resume browsing.
16-5982-82	100 ug
16-5982-85	500 ug

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
11-4317	Streptavidin FITC
11-4811	Anti-Rat IgG FITC
12-4317	Streptavidin PE
13-4813	Anti-Rat IgG Biotin (Polyclonal)
16-4321	Rat IgG2a K Isotype Control Functional Grade Purified
17-4317	Streptavidin APC

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
12-5982	Anti-Mouse CD274 (B7-H1) PE	488	575	FC	RUO
13-5982	Anti-Mouse CD274 (B7-H1) Biotin	N/A	N/A	FC	RUO
14-5982	Anti-Mouse CD274 (B7-H1) Purified	N/A	N/A	FA FC IHC (F) WB	RUO
16-5982	Anti-Mouse CD274 (B7-H1) Functional Grade* Purified	N/A	N/A	FA FC	RUO
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.

Description

The MIH5 monoclonal antibody reacts with mouse B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes including T, B, NK and DC. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH5 is reported to be a blocking antibody.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MIH5 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking in in vitro and in vivo functional assays.

Applications Tested

The MIH5 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Sheng H, Wang Y, Jin Y, Zhang Q, Zhang Y, Wang L, Shen B, Yin S, Liu W, Cui L, Li N. A critical role of IFNgamma in priming MSC-mediated suppression of T cell proliferation through up-regulation of B7-H1. Cell Res. 2008 Aug;18(8):846-57. (MIH5, FC, PubMed)

Saudemont A, Jouy N, Hetuin D, Quesnel B. NK cells that are activated by CXCL10 can kill dormant tumor cells that resist CTL-mediated lysis and can express B7-H1 that stimulates T cells. Blood. 2005 Mar 15;105(6):2428-35. (MIH5, FA, PubMed)

Schoop R, Wahl P, Le Hir M, Heemann U, Wang M, Wüthrich RP. Suppressed T-cell activation by IFN-gamma-induced expression of PD-L1 on renal tubular epithelial cells. Nephrol Dial Transplant. 2004 Nov;19(11):2713-20. (MIH5, FA, WB, IHC frozen, PubMed)

Aramaki O, Shirasugi N, Takayama T, Shimazu M, Kitajima M, Ikeda Y, Azuma M, Okumura K, Yagita H, Niimi M. Programmed death-1-programmed death-L1 interaction is essential for induction of regulatory cells by intratracheal delivery of alloantigen. Transplantation. 2004 Jan 15;77(1):6-12.

Kanai T, Totsuka T, Uraushihara K, Makita S, Nakamura T, Koganei K, Fukushima T, Akiba H, Yagita H, Okumura K, Machida U, Iwai H, Azuma M, Chen L, Watanabe M. Blockade of B7-H1 suppresses the development of chronic intestinal inflammation. J Immunol. 2003 Oct 15;171(8):4156-63.

Tsushima F, Iwai H, Otsuki N, Abe M, Hirose S, Yamazaki T, Akiba H, Yagita H, Takahashi Y, Omura K, Okumura K, Azuma M. Preferential contribution of B7-H1 to programmed death-1-mediated regulation of hapten-specific allergic inflammatory responses. Eur J Immunol. 2003 Oct;33(10):2773-82. (MIH5, FA, IHC frozen)

Freeman GJ, Long AJ, Iwai Y, Bourque K, Chernova T, Nishimura H, Fitz LJ, Malenkovich N, Okazaki T, Byrne MC, Horton HF, Fouser L, Carter L, Ling V, Bowman MR, Carreno BM, Collins M, Wood CR, Honjo T. Engagement of the PD-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation. J Exp Med. 2000 Oct 2;192(7):1027-34.

Dong H, Zhu G, Tamada K, Chen L. B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion. Nat Med. 1999 Dec;5(12):1365-9.

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Unless indicated otherwise, all products are For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for further distribution without written consent. Not all products available in all regions.