human BTLA antibody, Functional Grade Purified conjugate

Contents: Functional Grade Purified anti-human BTLA (CD272, B and T lymphocyte attenuator)
Catalog Number: 16-5979
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENT STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: MIH26
Isotype: Mouse IgG2a

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16-5979-82

100 ug

Staining of normal human peripheral blood cells with .25 μg of Purified Mouse IgG2a, K Iso Cntrl (cat. 14-4724) (open histogram) or .25 μg of FG Purified anti-human BTLA (MIH26) (colored histogram) followed by FITC Anti-Mouse IgG (cat. 11-4011). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5979	Affinity Purified anti-human BTLA (CD272, B and T lymphocyte attenuator)	N/A	N/A	FC
16-5979	Functional Grade* Purified anti-human BTLA (CD272, B and T lymphocyte attenuator)	N/A	N/A	FC
13-5979	Biotin anti-human BTLA (CD272, B and T lymphocyte attenuator)	N/A	N/A	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The BTLA monoclonal antibody reacts with human B and T lymphocyte attenuator (BTLA). BTLA is expressed by peripheral lymphocytes, splenic macrophages, developing B cells in the bone marrow and developing T cells in the thymus and mature, but not immature bone marrow-derived dendritic cells. BTLA is implicated as a negative regulator of the activation and/or function of various hemopoietic cell types. It is reported that BTLA binds to B7-H4, but further studies are needed to confirm this interaction.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This MIH26 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This MIH26 antibody has been tested by flow cytometric analysis of normal human peripheral blood. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Watanabe N, Gavrieli M, Sedy JR, Yang J, Fallarino F, Loftin SK, Hurchla MA, Zimmerman N, Sim J, Zang X, Murphy TL, Russell JH, Allison JP, Murphy KM. BTLA is a lymphocyte inhibitory receptor with similarities to CTLA-4 and PD-1. Nat Immunol. 2003 Jul;4(7):670-9.

Han P, Goularte OD, Rufner K, Wilkinson B, Kaye J. An inhibitory Ig superfamily protein expressed by lymphocytes and APCs is also an early marker of thymocyte positive selection. J Immunol. 2004 May 15;172(10):5931-9.

Sedy JR, Gavrieli M, Potter KG, Hurchla MA, Lindsley RC, Hildner K, Scheu S, Pfeffer K, Ware CF, Murphy TL, Murphy KM. B and T lymphocyte attenuator regulates T cell activation through interaction with herpesvirus entry mediator. Nat Immunol. 2005 Jan;6(1):90-8.

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Cat. 13-4013  Biotin Anti-Mouse IgG (clone Polyclonal)
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Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 16-4724  Functional Grade Purified Mouse IgG2a, K Isotype Control