Product Information

Contents: Functional Grade Purified anti-mouse B7-H3 (CD276, B7H3)
Catalog Number: 16-5973
Sizes: 50 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: M3.2D7
Isotype: Rat IgG2a

Staining of non-transfected (left) and mouse B7-H3 transfected (right) 293T cells with 0.25 μg of FG Purified Rat IgG2a Iso Cntrl (cat. 16-4321) (open histogram) or 0.25 μg of FG Purified M3.2D7 (colored histogram) followed by Biotin Anti-Rat IgG (cat. 13-4813)and SAv-PE (cat. 12-4317). Total cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5973	Purified anti-mouse/human CD276 (B7RP-2, B7H3)	N/A	N/A	FC
16-5973	Functional Grade* Purified anti-mouse/human CD276 (B7RP-2, B7H3)	N/A	N/A	FC
13-5973	Biotin anti-mouse/human CD276 (B7RP-2, B7H3)	N/A	N/A	FC
12-5973	Phycoerythrin (PE) anti-mouse/human CD276 (B7RP-2, B7H3)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The M3.2D7 monoclonal antibody was generated against and reacts with mouse B7-H3, a member of the B7 family of the Ig superfamily proteins. B7-H3 is reported to be expressed by antigen presenting cells and developing bone during embryogenesis. The ligand for B7-H3 has not yet been identified and it is thought that B7-H3 down-regulates T cell functions by engaging an unknown counter receptor on T cells. M3.2D7 stains mouse B7-H3 transfected cells and not mouse spleen cells. M3.2D7 also stains human B7-H3 transfected cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The M3.2D7 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The M3.2D7 antibody has been tested by flow cytometric analysis of mB7-H3 transfected cells. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Related Products

Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 16-4321    Functional Grade Purified Rat IgG2a Isotype Control
Cat. 11-4811    FITC Anti-Rat IgG
Cat. 13-4813    Biotin Anti-Rat IgG (clone Polyclonal)