Product Information

Contents: Functional Grade Purified anti-mouse B7S1 (B7H4, B7-H4, B7-S1, B7x)
Catalog Number: 16-5972
Sizes: 50 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: 188
Isotype: Rat IgG2b, κ

Staining of non-transfected (left) and mouse B7-H4 transfected (right) L5178Y cells with 0.25 μg FG Purified Rat IgG2b Iso Cntrl (cat. 16-4031) (open histogram) or 0.25 μg FG Purified anti-mouse B7S1/B7H4 (188) (colored histogram) followed by Biotin Anti-Rat IgG (cat. 13-4813)and Streptavidin-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5972	Purified anti-mouse B7-H4 (B7H4, B7S1, B7-S1, B7x)	N/A	N/A	FC
16-5972	Functional Grade* Purified anti-mouse B7-H4 (B7H4, B7S1, B7-S1, B7x)	N/A	N/A	FA FC
13-5972	Biotin anti-mouse B7-H4 (B7H4, B7S1, B7-S1, B7x)	N/A	N/A	FC
12-5972	Phycoerythrin (PE) anti-mouse B7-H4 (B7H4, B7S1, B7-S1, B7x)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 188 monoclonal antibody was generated against and reacts with mouse B7-H4 also known as B7S1, B7X. Cross reactivity of this antibody to other proteins has not been determined. B7-H4 is a newly discovered member of the B7 family reported to inhibit T cell activation, cell cycle progression and IL-2 production. The ligand for B7-H4 has not been identified yet. Simultaneous double staining of cells with two anti-mouse B7-H4 antibodies, Clone 9 and 188, suggests that epitopes recognized by these mAbs are different and/or there is no steric hindrance when antibodies are used together. 188 stains mouse B7-H4 transfected cells and not spleen cells. Exact expression pattern of B7-H4 has not been fully characterized.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 188 antibody has been reported for use in flow cytometric analysis and has also been reported to block binding of B7-H4 ligand in vitro.

Applications Tested

The 188 antibody has been tested by flow cytometric analysis of transfected cells. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Prasad DV, Richards S, Mai XM, Dong C. 2003. B7S1, a novel B7 family member that negatively regulates T cell activation. Immunity. 18(6):863-73.

Choi IH, Zhu G, Sica GL, Strome SE, Cheville JC, Lau JS, Zhu Y, Flies DB, Tamada K, Chen L. 2003. Genomic organization and expression analysis of B7-H4, an immune inhibitory molecule of the B7 family. J Immunol. 171(9):4650-4.

Sica GL, Choi IH, Zhu G, Tamada K, Wang SD, Tamura H, Chapoval AI, Flies DB, Bajorath J, Chen L. 2003. B7-H4, a molecule of the B7 family, negatively regulates T cell immunity. Immunity. 18(6):849-61.

Chen Y, Yang C, et al. 2006. Expression of the novel co-stimulatory molecule B7-H4 by renal tubular epithelial cells. Kidney Int. 2006 Dec;70(12):2092-9. (FA, PubMed)

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