Product Information

Contents: Functional Grade Purified anti-mouse TCRβ
Catalog Number: 16-5961
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: H57-597
Isotype: Armenian Hamster IgG

Surface staining of mouse splenocytes with anti-mouse TCR beta (H57-597) FITC (left), and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-5961	FITC anti-mouse TCR beta (TCRb)	488	518	FC
12-5961	PE anti-mouse TCR beta (TCRb)	488	575	FC
13-5961	Biotin anti-mouse TCR beta (TCRb)	N/A	N/A	FC
14-5961	Affinity Purified anti-mouse TCR beta (TCRb)	N/A	N/A	FA FC IHC
15-5961	PE-Cy5 anti-mouse TCR beta (TCRb)	488	670	FC
16-5961	Functional Grade* Purified anti-mouse TCR beta (TCRb)	N/A	N/A	FA FC
17-5961	APC anti-mouse TCR beta (TCRb)	633	660	FC
27-5961	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse TCR beta (TCRb) (APC-Cy7 Equivalent)	633	779	FC
35-5961	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse TCR beta (TCRb)	488	690	FC
36-5961	Functional Grade* Biotin anti-mouse TCR beta (TCRb)	N/A	N/A	FA FC IHC
45-5961	PerCP-Cy5.5 anti-mouse TCR beta (TCRb)	488	690	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The H57-597 monoclonal antibody reacts with the β chain of mouse TCR. TCRβ is expressed by thymocytes in a developmentally regulated manner and a majority of peripheral T cells. Crosslinking of the TCR complex with H57-597 induces activation and proliferation of T cells or apoptosis based on assay conditions. H57-597 is used as a phenotypic marker for TCR β expressing T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This H57-597 antibody has been reported for use in flow cytometric analysis. The H57-597 monoclonal antibody has also been reported for cross-linking the T-cell receptor (TCR) resulting in T-cell activation, and for in vitro depletion.

Applications Tested

The H57-597 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kubo, R. T., W. Born, et al. (1989). "Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors." J Immunol 142(8): 2736-42.

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