Anti-Mouse MHC Class I (H-2Kd) Functional Grade Purified

Also known as: MHC I, H2Kd, H2-Kd, H2K-d

RUO: For Research Use Only

Contents: Anti-Mouse MHC Class I (H-2Kd) Functional Grade Purified
Catalog Number: 16-5957
Concentration: 1 mg/mL
Formulation: aqueous buffer, no sodium azide
Storage Conditions: Store at 2-8°C.
Handling Conditions: Use in sterile environment.
Clone: SF1-1.1.1
Host/Isotype: Mouse IgG2a

Product Options
Cat. No.	Size	Price	Add Qty to Cart
16-5957-82	100 ug	To view relevant pricing, please select your country and resume browsing.
16-5957-85	500 ug

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
16-4724	Mouse IgG2a K Isotype Control Functional Grade Purified

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
12-5957	Anti-Mouse MHC Class I (H-2Kd) PE	488	575	FC	RUO
13-5957	Anti-Mouse MHC Class I (H-2Kd) Biotin	N/A	N/A	FC	RUO
16-5957	Anti-Mouse MHC Class I (H-2Kd) Functional Grade* Purified	N/A	N/A	FA FC	RUO
17-5957	Anti-Mouse MHC Class I (H-2Kd) APC	633	660	FC	RUO
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.

Description

This SF1-1.1.1 monoclonal antibody reacts with the H-2Kd MHC class I alloantigen. H-2Kd is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g. b, j, k, p, q, s, and v) has not been observed.

This monoclonal antibody has been reported to block CD8 involvement in TCR-ligand binding.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This SF1-1.1.1 antibody has been reported for use in functional assays.

Applications Tested

This SF1-1.1.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Noun G, Reboul M, Abastado JP, Jaulin C, Kourilsky P, Pla M. Alloreactive monoclonal antibodies select Kd molecules with different peptide profiles. J Immunol. 1996 Sep 15;157(6):2455-61. (SF1-1.1.1, FC and ELISA, Pubmed)

Renard V, Romero P, Vivier E, Malissen B, Luescher IF. CD8 beta increases CD8 coreceptor function and participation in TCR-ligand binding. J Exp Med. 1996 Dec 1;184(6):2439-44. (SF1-1.1.1, FA, Pubmed)

Luescher IF, Vivier E, Layer A, Mahiou J, Godeau F, Malissen B, Romero P. CD8 modulation of T-cell antigen receptor-ligand interactions on living cytotoxic T lymphocytes. Nature. 1995 Jan 26;373(6512):353-6. (SF1-1.1.1, FA, Pubmed)

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Unless indicated otherwise, all products are For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for further distribution without written consent. Not all products available in all regions.