Product Information

Contents: Functional Grade Purified anti-mouse IFNAR1 (IFNa/B Receptor 1)
Catalog Number: 16-5945
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, No NaN3
Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles. KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: MAR1-5A3
Isotype: Mouse IgG1

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
16-5945	Functional Grade* Purified anti-mouse IFNAR1 (IFNa/B Receptor 1)	N/A	N/A	NU
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

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Description

The MAR1-5A3 monoclonal antibody reacts with mouse IFN-a/b receptor subunit 1 (IFNAR-1). The type I IFN receptor subunit 1 is co-expressed with IFNAR-2 on nearly all cells and make up the heterodimeric receptor complex that binds to all type I IFNs, IFN-a and IFN-ß. Type I IFNs are a group of structurally and functionally related proteins that demonstrate antiviral, antiparasitic, and antiproliferative activities.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MAR1-5A3 antibody has been reported for use in neutralization of IFNAR1 bioactivity.

Applications Tested

The Functional Grade Purified MAR1-5A3 antibody has been tested by LAL assay to verify low endotoxin levels, by bioassay for neutralization of mouse IFNAR-1bioactivity. The MAR1-5A3 antibody at 0.04 ug/ml has been found to inhibit by 50% the biological effects of 0.5 ng/ml mouse IFN-a2 (ND50), in an EMCV assay of L929 cell protection. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in BestPortocols

References

K. C. Lai, et al. (2006) Blocking Monoclonal Antibodies Specific for Mouse IFN-a/b Receptor Subunit 1 (IFNAR-1) from Mice Immunized by In Vivo Hydrodynamic Transfection. J. INTERFERON AND CYTOKINE RESEARCH. 26: 804-819

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