Product Information

Contents: Functional Grade Purified anti-mouse NK1.1
Catalog Number: 16-5941
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: PK136
Isotype: Mouse IgG2a, κ

Two-color surface staining of C57BL/6 mouse splenocytes with anti-mouse NK-1.1 (PK136) PE and anti-mouse pan-NK cells (DX5) FITC. Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5941	Affinity Purified anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55)	N/A	N/A	FC IP
16-5941	Functional Grade* Purified anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55)	N/A	N/A	FC
13-5941	Biotin anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55)	N/A	N/A	FC
11-5941	FITC anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55)	488	518	FC
12-5941	PE anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55)	488	575	FC
17-5941	APC anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55)	633	660	FC
25-5941	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55)	488	760	FC
57-5941	Pacific Blue® anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55)	402	421	FC
45-5941	PerCP-Cy5.5 anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55) (PE-Cy5.5 replacement)	488	690	FC
95-5941	eFluor™ 650^NC anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55)	350-500	650	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in the NK1.1 mouse strains including C57BL and NZB. Several commonly used laboratory mouse strains such as BALB/c, SJL, AKR, CBA, C3H and A do not express the NK1.1 antigen. For detection of NK cells in these strains the monoclonal antibody DX5 (Cat. No. 14-5971) should be used. Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 reveals coexpression of both markers by a majority of cells as well as presence of small populations of DX5⁺PK136^- and DX5^-PK136⁺ cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. PK136 has been reported for use in flow cytometric analysis, depletion of NK cells, and in vitro functional studies.

Applications Tested

The PK136 antibody has been tested by flow cytometric analysis of C57BL/6 mouse splenocyte suspensions and can be used at less than or equal to 0.5 µg/million cells. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Koo, G. C. and J. R. Peppard (1984). "Establishment of monoclonal anti-Nk-1.1 antibody." Hybridoma 3(3): 301-3.

Kitaichi N, Kotake S, Morohashi T, Onoe K, Ohno S, Taylor AW. Diminution of experimental autoimmune uveoretinitis (EAU) in mice depleted of NK cells. J Leukoc Biol. 2002 Dec;72(6):1117-21. (PK136, in vivo depletion, PubMed)

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