Product Information

Contents: Functional Grade Purified anti-mouse CD95 Ligand (Fas Ligand, CD178)
Catalog Number: 16-5912
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: MFL4
Isotype: Armenian Hamster IgG

Surface staining of mouse Fas Ligand transfected cells with anti-mouse/rat CD178 (MFL4) Purified followed by anti-armenian hamster IgG Biotin and SA-PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5912	Affinity Purified anti-mouse/rat CD95 Ligand (Fas Ligand, CD178, FasL)	N/A	N/A	FA FC
16-5912	Functional Grade* Purified anti-mouse/rat CD95 Ligand (Fas Ligand, CD178, FasL)	N/A	N/A	FA FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The MFL4 monoclonal antibody reacts with mouse and rat Fas Ligand (CD178), a 40 kDa type II transmembrane glycoprotein. FasL is a member of the TNF family and is expressed by activated T cells. The interaction of FasL with its receptor CD95 induces Fas-mediated killing. It has been reported that the human FasL antigen is cleaved from the surface by matrix metalloproteinases (MMPs), resulting in a 26 kDa soluble form. The degree of sensitivity for the mouse and rat antigens to MMPs has not been reported. MFL4 has been reported in the literature to cross-react with the rat FasL antigen.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MFL4 antibody has been reported for use in flow cytometric analysis. MFL4 has also been reported in blocking of FasL mediated killing in functional assays.

Applications Tested

The MFL4 antibody has been tested by flow cytometric analysis of mouse FasL transfected cells and activated T cells. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kayagaki, N., N. Yamaguchi, et al. 1997. Polymorphism of murine Fas ligand that affects the biological activity. Proc Natl Acad Sci U S A 94(8): 3914-9.
Nakajima, A., H. Hirai, et al. 2000. Treatment of lupus in NZB/W F1 mice with monoclonal antibody against fas ligand. J Autoimmun 14(2): 151-7.
Kayagaki, N., A. Kawasaki, et al. 1995. Metalloproteinase-mediated release of human Fas ligand. J Exp Med 182(6): 1777-83.
Trinite, B., C. Voisine, et al. 2000. A subset of cytolytic dendritic cells in rat. J. Immunol. 165(8): 4202-8

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