Product Information

Contents: Functional Grade Purified anti-mouse CD95 Ligand (Fas Ligand, CD178)
Catalog Number: 16-5911
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: MFL3
Isotype: Armenian Hamster IgG

Surface staining of mouse Fas Ligand transfected cells with anti-mouse CD178 (MFL3) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-5911	PE anti-mouse CD95 Ligand (Fas Ligand, CD178, FasL)	488	575	FC
13-5911	Biotin anti-mouse CD95 Ligand (Fas Ligand, CD178, FasL)	N/A	N/A	FC
14-5911	Affinity Purified anti-mouse CD95 Ligand (Fas Ligand, CD178, FasL)	N/A	N/A	FA FC
16-5911	Functional Grade* Purified anti-mouse CD95 Ligand (Fas Ligand, CD178, FasL)	N/A	N/A	FA FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The MFL3 monoclonal antibody reacts with mouse Fas (CD95) Ligand, a 40 kDa type II transmembrane glycoprotein. FasL is a member of the TNF family and is expressed by mouse activated T cells. The interaction of FasL with its receptor CD95 induces Fas-mediated killing. It has been reported that the human FasL antigen is cleaved from the surface by matrix metalloproteinases (MMPs), resulting in a 26 kDa soluble form. The degree of sensitivity for the mouse antigen to MMPs has not been reported.

Applications Reported

For research use only, not for diagnostic or therapeutic use. MFL3 has been reported for use in flow cytometric analysis. It has also been reported in blocking of the FasL mediated killing in functional assays.

Applications Tested

The MFL3 antibody has been tested by flow cytometric analysis of mouse FasL transfected cells and activated T cells. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kayagaki, N., N. Yamaguchi, et al. (1997). "Polymorphism of murine Fas ligand that affects the biological activity." Proc Natl Acad Sci U S A 94(8): 3914-9.
Nakajima, A., H. Hirai, et al. (2000). "Treatment of lupus in NZB/W F1 mice with monoclonal antibody against fas ligand." J Autoimmun 14(2): 151-7.
Kayagaki, N., A. Kawasaki, et al. (1995). "Metalloproteinase-mediated release of human Fas ligand." J Exp Med 182(6): 1777-83.

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