human FceRI alpha antibody, Functional Grade Purified conjugate

Contents: Functional Grade Purified anti-human FceRI alpha (FceRIa, FceRI-a, FceRI-alpha, high affinity IgE receptor)
Catalog Number: 16-5899
Formulation: Phosphate buffered saline, pH 7.2, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENT STERILE.
Clone: AER-37 (CRA1)
Isotype: Mouse IgG2b, κ

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16-5899-82

100 ug

Staining of human lysed whole blood with Purified Mouse IgG2b Iso Cntrl (cat. 14-4732) (green histogram) or Purified AER-37 (CRA1) (blue histogram) followed by Biotin Anti-Mouse IgG (cat. 13-4013)and Streptavidin-PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5899	Affinity Purified anti-human FceRI alpha (FceRIa, FceRI-a, FceRI-alpha, high affinity IgE receptor)	N/A	N/A	FC
16-5899	Functional Grade* Purified anti-human FceRI alpha (FceRIa, FceRI-a, FceRI-alpha, high affinity IgE receptor)	N/A	N/A	FC
13-5899	Biotin anti-human FceRI alpha (FceRIa, FceRI-a, FceRI-alpha, high affinity IgE receptor)	N/A	N/A	FC
11-5899	Fluorescein isothiocyanate (FITC) anti-human FceRI alpha (FceRIa, FceRI-a, FceRI-alpha, high affinity IgE receptor)	488	518	FC
12-5899	PE anti-human FceRI alpha (FceRIa, FceRI-a, FceRI-alpha, high affinity IgE receptor)	488	575	FC
17-5899	Allophycocyanin (APC) anti-human FceRI alpha (FceRIa, FceRI-a, FceRI-alpha, high affinity IgE receptor)	633	660	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The AER-37 monoclonal antibody reacts with the FcεRIα subunit, an IgE-binding subunit lacking signal-transducing ability. FcεRIα is expressed on mast and basophil cells and is upregulated by the presence of IgE. FcεRIα forms a tetrameric complex with one β and two γ subunits. The β and γ subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The FcεRI complex plays an important role in triggering IgE-mediated allergic reactions.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This AER-37 (CRA1) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This AER-37 (CRA1) antibody has been tested by flow cytometric analysis of peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Hakimi J., C. Seals, J. A. Kondas, L. Pettine, W. Danho, J. Kochan. (1990) The Alpha Subunit of the Human IgG Receptor (FceRI) is Sufficient for High-Affinity IgE Binding. J Biol Chem 265(36):22079-81

Hasegawa S, Pawankar R, Suzuki K, Nakahata T, Furukawa S, Okumura K, Ra C. (1999) Functional expression of the high affinity receptor for IgE (FcepsilonRI) in human platelets and its' intracellular expression in human megakaryocytes. Blood 93(8):2543-51.

Hasegawa M, Nishiyama C, Nishiyama M, Akizawa Y, Takahashi K, Ito T, Furukawa S, Ra C, Okumura K, Ogawa H. (2003) Regulation of the human Fc(epsilon)RI alpha-chain distal promoter. J Immunol 170(7):3732-8.

Yamaguchi M, Sayama K, Yano K, Lantz CS, Noben-Trauth N, Ra C, Costa JJ, Galli SJ. IgE enhances Fc epsilon receptor I expression and IgE-dependent release of histamine and lipid mediators from human umbilical cord blood-derived mast cells: synergistic effect of IL-4 and IgE on human mast cell Fc epsilon receptor I expression and mediator release. J Immunol. 1999 May 1;162(9):5455-65. (AER-37, FC, PubMed)

Suzukawa M, Hirai K, Iikura M, Nagase H, Komiya A, Yoshimura-Uchiyama C, Yamada H, Ra C, Ohta K, Yamamoto K, Yamaguchi M. IgE- and FcepsilonRI-mediated migration of human basophils. Int Immunol. 2005 Sep;17(9):1249-55. (AER-37, FA, PubMed)

Ra C, Kuromitsu S, Hirose T, Yasuda S, Furuichi K, Okumura K. Soluble human high-affinity receptor for IgE abrogates the IgE-mediated allergic reaction. Int Immunol. 1993 Jan;5(1):47-54.

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