Product Information

Contents: Functional Grade Purified anti-mouse FcεRIα (FceRI-α, high affinity IgE receptor)
Catalog Number: 16-5898
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: MAR-1
Isotype: Armenian Hamster IgG

Staining of MC/9 cells with 0.125 μg of FG Purified Armenian Hamster IgG Iso Cntrl (cat. 16-4444) (open histogram) or 0.125 μg of FG Purified MAR-1 (colored histogram) followed by FITC Anti-Armenian Hamster IgG (cat. 11-4111). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5898	Affinity Purified anti-mouse FceRI alpha (FceRIa, FceRI-a, FceRI-alpha, high affinity IgE receptor)	N/A	N/A	FA FC IH/F IP WB
16-5898	Functional Grade* Purified anti-mouse FceRI alpha (FceRIa, FceRI-a, FceRI-alpha, high affinity IgE receptor)	N/A	N/A	FA FC
13-5898	Biotin anti-mouse FceRI alpha (FceRIa, FceRI-a, FceRI-alpha, high affinity IgE receptor)	N/A	N/A	FC
11-5898	FITC anti-mouse FceRI alpha (FceRIa, FceRI-a, FceRI-alpha, high affinity IgE receptor)	488	518	FC
12-5898	PE anti-mouse FceRI alpha (FceRIa, FceRI-a, FceRI-alpha, high affinity IgE receptor)	488	575	FC
51-5898	Alexa Fluor® 647 anti-mouse FceRI alpha (FceRIa, FceRI-a, FceRI-alpha, high affinity IgE receptor)	633	668	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The MAR-1 monoclonal antibody reacts with the FcεRIα subunit, an IgE-binding subunit lacking signal-transducing ability. FcεRIα is expressed on mast and basophil cells and is up-regulated by the presence of IgE. FcεRIα forms a tetrameric complex with one β and two γ subunits. The β and γ subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The FcεRI complex plays an important role in triggering IgE-mediated allergic reactions.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MAR-1 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of IgE to Fcε Receptor and degranulation of mast cells.

Applications Tested

The MAR-1 antibody has been tested by flow cytometric analysis of the MC/9 cell line (a mouse mast cell line). This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Arinobu Y, Iwasaki H, Gurish MF, Mizuno S, Shigematsu H, Ozawa H, Tenen DG, Austen KF, Akashi K. Developmental checkpoints of the basophil/mast cell lineages in adult murine hematopoiesis. Proc Natl Acad Sci U S A. 2005 Dec 13;102(50):18105-10. Epub 2005 Dec 5.(mar-1, FC, PubMed)

Obata K, Mukai K, Tsujimura Y, Ishiwata K, Kawano Y, Minegishi Y, Watanabe N, Karasuyama H. Basophils are essential initiators of a novel type of chronic allergic inflammation. Blood. 2007 Aug 1;110(3):913-20. Epub 2007 Apr 4 (Mar-1, FC)

Sokol CL, Barton GM, Farr AG, Medzhitov R. A mechanism for the initiation of allergen-induced T helper type 2 responses. Nat Immunol. 2008 Mar;9(3):310-8.(Mar-1, FA, PubMed)

Yamaguchi M., K. Hirai, A. Komiya, M. Miyamasu, Y. Furumoto, R. Teshima, K. Ohta, Y. Morita, S. J. Galli, C. Ra, K. Yamamoto. (2001) Regulation of Mouse Mast Cell Surface Fc epsilon RI expression by dexamethasone. Int Immunol 13(7):843-51.

Related Products

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Cat. 13-4113    Biotin Anti-Armenian Hamster IgG (clone Polyclonal)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 16-4888    Functional Grade Purified Armenian Hamster IgG Isotype Control (clone eBio299Arm)