Staining of C57Bl/6 splenocytes with 0.5 µg of Rat IgG2a Isotype Control Purified (cat.14-4321) (open histogram) or 0.125 µg of Anti-Mouse NKG2A/C/E Purified (filled histogram) followed by Anti-Rat IgG FITC (cat.11-4811). Total viable cells were used for analysis.
Contents: Anti-Mouse NKG2A/C/E Functional Grade Purified Catalog Number: 16-5896 Concentration: 1 mg/ml Formulation: Phosphate buffered saline, pH 7.2, No NaN3 Storage Conditions: Store at 2-8°C. Handling Conditions: Use in sterile envrioment. Clone: 20d5 Host/Isotype: Rat IgG2a, κ
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16-5896-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The 20d5 monoclonal antibody reacts with the mouse NKG2A, C, and E. NKG2 molecules belong to a C-type lectin-like family of cell surface receptors expressed by mouse NK and NKT cell lineages. NKG2 molecules form heterodimeric complexes with CD94 and are responsible for recognition of non-classical MHC class I antigen Qa-1.
When co-staining with NKG2AB6clone 16a11, it is important to stain for NKG2AB6 first then subsequently with NKG2A/C/E, as steric hindrance has been observed if co-stained concurrently.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 20d5 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This 20d5 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Fraser KP, Gays F, Robinson JH, van Beneden K, Leclercq G, Vance RE, Raulet DH, Brooks CG. 2002. NK cells developing in vitro from fetal mouse progenitors express at least one member of the Ly49 family that is acquired in a time-dependent and stochastic manner independently of CD94 and NKG2. Eur J Immunol. 32:868-78. Vance RE, Jamieson AM, Cado D, Raulet DH. 2002. Implications of CD94 deficiency and monoallelic NKG2A expression for natural killer cell development and repertoire formation. Proc Natl Acad Sci U S A. 99:868-73. Kraft JR, Vance RE, Pohl J, Martin AM, Raulet DH, Jensen PE. 2000. Analysis of Qa-1(b) peptide binding specificity and the capacity of CD94/NKG2A to discriminate between Qa-1-peptide complexes. J Exp Med. 192:613-24. Vance RE, Jamieson AM, Raulet DH. 1999. Recognition of the class Ib molecule Qa-1(b) by putative activating receptors CD94/NKG2C and CD94/NKG2E on mouse natural killer cells. J Exp Med. 190:1801-12. Vance RE, Kraft JR, Altman JD, Jensen PE, Raulet DH.1998. Mouse CD94/NKG2A is a natural killer cell receptor for the nonclassical major histocompatibility complex (MHC) class I molecule Qa-1(b). J Exp Med. 188:1841-8. Vance RE, Tanamachi DM, Hanke T, Raulet DH.1997. Cloning of a mouse homolog of CD94 extends the family of C-type lectins on murine natural killer cells. Eur J Immunol. 27:3236-41
