Product Information

Contents: Functional Grade Purified anti-mouse Ly-49I
Catalog Number: 16-5895
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: YLI-90
Isotype: Mouse IgG1, κ

Two-color surface staining of mouse splenocytes with anti-mouse Ly-49I (YLI-90) FITC and anti-mouse CD3e (145-2C11) PE. Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5895	Affinity Purified anti-mouse Ly-49I	N/A	N/A	FC
16-5895	Functional Grade* Purified anti-mouse Ly-49I	N/A	N/A	FC
13-5895	Biotin anti-mouse Ly-49I	N/A	N/A	FC
11-5895	FITC anti-mouse Ly-49I	488	518	FC
12-5895	PE anti-mouse Ly-49I	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The YLI-90 monoclonal antibody reacts with the B6 mouse Ly-49I receptor. Ly49I, a member of the Ly49 family with inhibitory function, is a homodimer type II transmembrane protein and contains an Immunoreceptor Tyrosine-based Inhibitory Motif (ITIM). YLI-90 binds to subpopulations of NK, NKT and T cell lineages in the C57Bl/6 strain. This antibody does not bind to cells from BALB/c, SJL or AKR/J mice.

Applications Reported

For research use only, not for diagnostic or therapeutic use. YLI-90 has been reported for use in flow cytometric analysis.

Applications Tested

The YLI-90 antibody has been tested by flow cytometric analysis of C57Bl/6 and BALB/c mouse splenocyte suspensions and can be used at less than or equal to 0.25 μg/million cells. For optimal detection of Ly-49I using YLI-90, a fluorochrome conjugated isotype-specific second step is recommended. If using a polyclonal anti-mouse IgG second step, please note that splenic B cells will also stain due to binding of the second step to surface IgG. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Coles, M. C., C. W. McMahon, et al. 2000. Memory CD8 T lymphocytes express inhibitory MHC-specific Ly49 receptors. Eur J Immunol 30: 236-44.

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