Product Information

Contents: Functional Grade Purified anti-mouse KLRG1/MAFA
Catalog Number: 16-5893
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: 2F1
Isotype: Golden Syrian Hamster IgG

Surface staining of mouse splenocytes with anti-mouse KLRG1 (2F1) Biotin followed by SA-PE and anti-mouse NK-1.1 (PK136) APC. Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5893	Affinity Purified anti-mouse KLRG1/MAFA	N/A	N/A	FC IP
16-5893	Functional Grade* Purified anti-mouse KLRG1/MAFA	N/A	N/A	FA FC
13-5893	Biotin anti-mouse KLRG1/MAFA	N/A	N/A	FC
17-5893	Allophycocyanin (APC) anti-mouse KLRG1/MAFA	633	660	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 2F1 monoclonal antibody reacts with the mouse Killer cell Lectin-like Receptor G1 (KLRG1), also known as Mast cell Function-associated Antigen (MAFA). KLRG1 is a homodimer of glycosylated 30-38 kDa subunits and contains a cytoplasmic motif similar to the immunoreceptor tyrosine-based inhibitory motif (ITIM). Rat MAFA was identified as an antigen specific to rat mast cells, however, the expression of mouse KLRG1/MAFA with 2F1 has not been detected on the surface of mouse mast cell lines, bone marrow-derived mast cells, or peritoneal mast cells. This antigen is expressed on 30-60% of mouse NK cells and a subset of T cells. MHC class I molecules regulate KLRG1 via interactions with class I-specific inhibitory Ly49 molecules and SHP-1 signaling. Although KLRG1 and Ly49 molecules are both lectin-like inhibitory receptors that are regulated by class I expression, the effects of class I on the cell surface expression of the molecules are opposing, and the underlying regulatory mechanisms are distinct.

Applications Reported

For research use only, not for diagnostic or therapeutic use. 2F1 has been reported for use in flow cytometric analysis, and functional assays.

Applications Tested

The 2F1 antibody has been tested by flow cytometric analysis of mouse splenocyte cell suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Beyersdorf NB, Ding X, Karp K, Hanke T. 2001. Expression of inhibitory "killer cell lectin-like receptor G1" identifies unique subpopulations of effector and memory CD8 T cells. Eur J Immunol. 31:3443-52
Voehringer D, Blaser C, Brawand P, Raulet DH, Hanke T, Pircher H. 2001. Viral infections induce abundant numbers of senescent CD8 T cells. J Immunol. 167:4838-43
Corral L, Hanke T, Vance RE, Cado D, Raulet DH. 2000. NK cell expression of the killer cell lectin-like receptor G1 (KLRG1), the mouse homolog of MAFA, is modulated by MHC class I molecules. Eur J Immunol. 30:920-30

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