Product Information

Contents: Functional Grade Purified anti-mouse LPAM-1 (Integrin alpha 4 beta 7 complex)
Catalog Number: 16-5887
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffered saline, pH 7.2, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENT STERILE.
Clone: DATK32 (DATK-32)
Isotype: Rat IgG2a, κ

Staining of C57Bl/6 splenocytes with 0.5 μg of Purified Rat IgG2a Iso Cntrl (cat. 14-4321) (open histogram) or 0.5 μg of Purified anti-mouse LPAM-1 (DATK32) (colored histogram) followed by Biotin Anti-Rat IgG (cat. 13-4813)and Streptavidin-PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5887	Affinity Purified anti-mouse LPAM-1 (Integrin alpha 4 beta 7 complex)	N/A	N/A	FA FC
16-5887	Functional Grade* Purified anti-mouse LPAM-1 (Integrin alpha 4 beta 7 complex)	N/A	N/A	FA FC
13-5887	Biotin anti-mouse LPAM-1 (Integrin alpha 4 beta 7 complex)	N/A	N/A	FC
12-5887	Phycoerythrin (PE) anti-mouse LPAM-1 (Integrin alpha 4 beta 7 complex)	488	575	FC
17-5887	Allophycocyanin (APC) anti-mouse LPAM-1 (Integrin alpha 4 beta 7 complex)	633	660	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The DATK32 monoclonal antibody binds to a combinatorial epitope of mouse LPAM-1 (α₄β₇), which is a heterodimer of the the 154 kDa α₄ subunit and the 130 kDa β₇ subunit. In mouse, the β₇ subunit is found on the majority of mature lymphocytes and a small population of thymocytes and bone marrow cells. LPAM-1 is an integrin involved in the transendothelial migration of lymphocytes across high endothelial venules (HEV), and is know to bind several ligands including MAdCAM-1, VCAM-1 and fibronectin. Binding of the DATK32 monoclonal antibody has been shown to induce aggregation of the CD8+ T cell lymphoma TK1, and block LPAM-1-mediated cell adhesion.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This DATK32 (DATK-32) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This DATK32 (DATK-32) antibody has been tested tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Berlin C, Berg EL, Briskin MJ, Andrew DP, Kilshaw PJ, Holzmann B, Weissman IL, Hamann A, Butcher EC. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell. 1993 Jul 16;74(1):185-95. (DATK32, FC, FA, PubMed)

Andrew DP, Berlin C, Honda S, Yoshino T, Hamann A, Holzmann B, Kilshaw PJ, Butcher EC. Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. J Immunol. 1994 Nov 1;153(9):3847-61. (DATK32, FC, FA, PubMed)

Hamann A, Andrew DP, Jablonski-Westrich D, Holzmann B, Butcher EC. Role of alpha 4-integrins in lymphocyte homing to mucosal tissues in vivo. J Immunol. 1994 Apr 1;152(7):3282-93. (DATK32, FC, FA, PubMed)

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Cat. 16-4321 Functional Grade Purified Rat IgG2a Isotype Control