Product Information

Contents: Functional Grade Purified anti-mouse DR5 (CD262, Trail-R2, TrailR2)
Catalog Number: 16-5883
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: MD5-1
Isotype: Armenian Hamster IgG

Surface staining of DR5 transfected cells with anti-mouse DR5 (MD5-1) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5883	Affinity Purified anti-mouse DR5 (CD262, Trail-R2, TrailR2)	N/A	N/A	FA FC
16-5883	Functional Grade* Purified anti-mouse DR5 (CD262, Trail-R2, TrailR2)	N/A	N/A	FA FC
13-5883	Biotin anti-mouse DR5 (CD262, Trail-R2, TrailR2)	N/A	N/A	FC
12-5883	PE anti-mouse DR5 (CD262, Trail-R2, TrailR2)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The MD5-1 monoclonal antibody reacts with mouse DR5, also known as TRAIL-R2, Apo2, TRICK2, and KILLER. DR5 binds to TRAIL, activates NF-κB, and induces TRAIL-mediated apoptosis. DR5 mRNA is expressed broadly by normal tissue and human antigen is also expressed by some tumor cell lines.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MD5-1 antibody has been reported for use in flow cytometric analysis. It has also been reported for use in functional assays.

Applications Tested

The MD5-1 antibody has been tested by flow cytometric analysis of mouse DR5-transfected cells. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Sheridan, J. P., S. A. Marster, R. M. Pitti, A. Gurney, M. Skubatch, D. Baldwin, L. Ramakrishnan, C. L. Gray, K. Baker, W. I. Wood, A. D. Goddard, P. Godowski, A. Ashkenazi. 1997. Control of TRAIL-Induced Apoptosis by a Family of Signaling and Decoy Receptors. Science 277:818-821.
Takeda K, Yamaguchi N, Akiba H, Kojima Y, Hayakawa Y, Tanner JE, Sayers TJ, Seki N, Okumura K, Yagita H, Smyth MJ. 2004. Induction of Tumor-specific T Cell Immunity by Anti-DR5 Antibody Therapy. J Exp Med. Feb 16;199(4):437-48. Epub 2004 Feb 09.

Related Products

Cat. 16-4444  Functional Grade Purified Armenian Hamster IgG Isotype Control (clone n/a)
Cat. 16-4888  Functional Grade Purified Armenian Hamster IgG Isotype Control (clone eBio299Arm)
Cat. 14-6019  Anti-human DR5 (CD262, Trail-R2, TrailR2) polyclonal (clone Polyclonal)