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Product Information


Contents: Functional Grade Purified anti-mouse DR5 (CD262, Trail-R2, TrailR2)
Catalog Number: 16-5883
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN3
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: MD5-1
Isotype: Armenian Hamster IgG
 
 
data image 1
Surface staining of DR5 transfected cells with anti-mouse DR5 (MD5-1) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis. 

Available Formats of This Product
Cat. No. Format Excite
(nm)
Emit
(nm)
Reported Applications
14-5883 Affinity Purified anti-mouse DR5 (CD262, Trail-R2, TrailR2) N/A N/A FA  FC 
16-5883 Functional Grade* Purified anti-mouse DR5 (CD262, Trail-R2, TrailR2) N/A N/A FA  FC 
13-5883 Biotin anti-mouse DR5 (CD262, Trail-R2, TrailR2) N/A N/A FC 
12-5883 PE anti-mouse DR5 (CD262, Trail-R2, TrailR2) 488 575 FC 
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg  (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
  Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description


The MD5-1 monoclonal antibody reacts with mouse DR5, also known as TRAIL-R2, Apo2, TRICK2, and KILLER. DR5 binds to TRAIL, activates NF-κB, and induces TRAIL-mediated apoptosis. DR5 mRNA is expressed broadly by normal tissue and human antigen is also expressed by some tumor cell lines.


Applications Reported


For research use only, not for diagnostic or therapeutic use. The MD5-1 antibody has been reported for use in flow cytometric analysis. It has also been reported for use in functional assays.


Applications Tested


The MD5-1 antibody has been tested by flow cytometric analysis of mouse DR5-transfected cells. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.


References



Sheridan, J. P., S. A. Marster, R. M. Pitti, A. Gurney, M. Skubatch, D. Baldwin, L. Ramakrishnan, C. L. Gray, K. Baker, W. I. Wood, A. D. Goddard, P. Godowski, A. Ashkenazi. 1997. Control of TRAIL-Induced Apoptosis by a Family of Signaling and Decoy Receptors. Science 277:818-821.
Takeda K, Yamaguchi N, Akiba H, Kojima Y, Hayakawa Y, Tanner JE, Sayers TJ, Seki N, Okumura K, Yagita H, Smyth MJ. 2004. Induction of Tumor-specific T Cell Immunity by Anti-DR5 Antibody Therapy. J Exp Med. Feb 16;199(4):437-48. Epub 2004 Feb 09.


Related Products


Cat. 16-4444    Functional Grade Purified Armenian Hamster IgG Isotype Control (clone n/a)
Cat. 16-4888    Functional Grade Purified Armenian Hamster IgG Isotype Control (clone eBio299Arm)
Cat. 14-6019    Anti-human DR5 (CD262, Trail-R2, TrailR2) polyclonal (clone Polyclonal)



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