anti-mouse NKG2D (CD314) antibody: Functional Grade

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Contents: Functional Grade Purified anti-mouse NKG2D / CD314 (blocking)
Catalog Number: 16-5882
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.04 ng/ug antibody, as determined by the LAL assay.
Clone: CX5
Isotype: Rat IgG1, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
16-5882-81	50 ug	$90
16-5882-82	100 ug	$170
16-5882-85	500 ug	$280
16-5882-86	1 mg	$475

*International customers: Please contact your distributor for region specific pricing.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-5882	PE anti-mouse NKG2D / CD314 (blocking)	488	575	FC
13-5882	Biotin anti-mouse NKG2D / CD314 (blocking)	N/A	N/A	FC
14-5882	Affinity Purified anti-mouse NKG2D / CD314 (blocking)	N/A	N/A	FA FC
16-5882	Functional Grade* Purified anti-mouse NKG2D / CD314 (blocking)	N/A	N/A	FA FC
17-5882	APC anti-mouse NKG2D / CD314 (blocking)	633	660	FC
25-5882	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse NKG2D / CD314 (blocking)	488	760	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The CX5 monoclonal antibody reacts with the mouse NKG2D, a lectin-like molecule expressed on both human and mouse NK cells. Mouse NKG2D binds to retinoic acid-inducible RAE-1α, -β, -γ, -δ, -ε and the minor histocompatibility molecule H60 and has the ability to costimulate multiple NK activation receptors, through the DAP12/DAP10 adaptor molecules. NKG2D is expressed by all spleen and liver NK cells, NK1.1⁺ thymocytes, in vitro activated LAK cells, and a subset of splenic NKT cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The CX5 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of binding of NKG2D to its ligands, RAE-1 and H60, and inhibition NKG2D-dependent NK cell-mediated cytotoxicity against NKG2D ligand-bearing tumors in vitro and in vivo.

Applications Tested

The CX5 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that when using this antibody for staining the secondary must be Biotin anti-rat (cat 13-4813). Other secondaries may give suboptimal staining. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Lodoen M, Ogasawara K, Hamerman JA, Arase H, Houchins JP, Mocarski ES, Lanier LL. 2003. NKG2D-mediated natural killer cell protection against cytomegalovirus is impaired by viral gp40 modulation of retinoic acid early inducible 1 gene molecules. J Exp Med. 197(10):1245-53.
Cerwenka A, Baron JL, Lanier LL. 2001. Ectopic expression of retinoic acid early inducible-1 gene (RAE-1) permits natural killer cell-mediated rejection of a MHC class I-bearing tumor in vivo. Proc Natl Acad Sci U S A. 98(20):11521-6.
Cerwenka A, Bakker AB, McClanahan T, Wagner J, Wu J, Phillips JH, Lanier LL. 2000. Retinoic acid early inducible genes define a ligand family for the activating NKG2D receptor in mice. Immunity. 12(6):721-7.

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