Product Information

Contents: Functional Grade Purified anti-mouse NKG2D / CD314 (blocking)
Catalog Number: 16-5880
Sizes: 50 ug, 100 ug, 500 ug, 1 mg
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENT STERILE.
Clone: MI-6 (MI6)
Isotype: Rat IgG2a, λ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5880	Affinity Purified anti-mouse NKG2D / CD314 (blocking)	N/A	N/A	FA FC
16-5880	Functional Grade* Purified anti-mouse NKG2D / CD314 (blocking)	N/A	N/A	FA FC
13-5880	Biotin anti-mouse NKG2D / CD314 (blocking)	N/A	N/A	FA FC
51-5880	Alexa Fluor® 647 anti-mouse NKG2D / CD314 (blocking)	633	668	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The MI-6 monoclonal antibody reacts with the mouse NKG2D, a lectin-like molecule expressed on both human and mouse NK cells. Mouse NKG2D binds to retinoic acid-inducible RAE-1α, -β, -γ, -δ, -ε and the minor histocompatibility molecule H60 and has the ability to costimulate multiple NK activation receptors, through the DAP12/DAP10 adaptor molecules. NKG2D is expressed by all spleen and liver NK cells, NK1.1⁺ thymocytes, in vitro activated LAK cells, and a subset of splenic NKT cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This MI-6 (MI6) antibody has been reported for use in flow cytometric analysis. Furthermore, engagement of NKG2D with the MI-6 monoclonal antibody has been demonstrated to block NKG2D-mediated cytolysis, both in vitro and in vivo.

Applications Tested

This MI-6 (MI6) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Jamieson AM, Diefenbach A, McMahon CW, Xiong N, Carlyle JR, Raulet DH. The role of the NKG2D immunoreceptor in immune cell activation and natural killing. Immunity. 2002 Jul;17(1):19-29. Erratum in: Immunity. 2004 Jun;20(6):799. (MI-6, FC, FA, PubMed)

Diefenbach A, Hsia J, Hsiung M, Raulet D. 2003. A novel ligand for the NKG2D receptor activates NK cells and macrophages and induces tumor immunity. Eur. J. Immunol. 33(2):381 - 391.

Lodoen M, Ogasawara K, Hamerman JA, Arase H, Houchins JP, Mocarski ES, Lanier LL. 2003. NKG2D-mediated natural killer cell protection against cytomegalovirus is impaired by viral gp40 modulation of retinoic acid early inducible 1 gene molecules. J Exp Med. 197(10):1245-53.

Cerwenka A, Baron JL, Lanier LL. 2001. Ectopic expression of retinoic acid early inducible-1 gene (RAE-1) permits natural killer cell-mediated rejection of a MHC class I-bearing tumor in vivo. Proc Natl Acad Sci U S A. 98(20):11521-6.

Cerwenka A, Bakker AB, McClanahan T, Wagner J, Wu J, Phillips JH, Lanier LL. 2000. Retinoic acid early inducible genes define a ligand family for the activating NKG2D receptor in mice. Immunity. 12(6):721-7.

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