Product Information

Contents: Functional Grade Purified anti-mouse GITR (TNFRSF18)
Catalog Number: 16-5874
Sizes: 50 ug, 200 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: DTA-1
Isotype: Rat IgG2b

Staining of C57Bl/6 splenocytes with .015 μg of Purified Rat IgG2b Isotype Control (cat. 14-4031) (open histogram) or .015 μg of Purified anti-mouse GITR (DTA-1) (colored histogram) followed by Biotin Anti-Rat IgG (cat. 13-4813)and Streptavidin-PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5874	Affinity Purified anti-mouse GITR (TNFRSF18, AITR)	N/A	N/A	FA FC IP
16-5874	Functional Grade* Purified anti-mouse GITR (TNFRSF18, AITR)	N/A	N/A	FA FC
13-5874	Biotin anti-mouse GITR (TNFRSF18, AITR)	N/A	N/A	FC
11-5874	Fluorescein isothiocyanate (FITC) anti-mouse GITR (TNFRSF18, AITR)	488	518	FC
12-5874	Phycoerythrin (PE) anti-mouse GITR (TNFRSF18, AITR)	488	575	FC
17-5874	Allophycocyanin (APC) anti-mouse GITR (TNFRSF18, AITR)	633	660	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The DTA-1 monoclonal antibody reacts with mouse GITR, Glucocorticoid-Induced TNFR family gene, also known as TNFRSF18. In naïve mice, GITR is expressed predominantly by CD4⁺CD25⁺ T regulatory cells (Treg) and by CD25⁺ CD4⁺ CD8^- thymocytes. Stimulation of GITR abrogates Treg cell-mediated suppression. The removal of GITR-expressing Treg cells or the administration of DTA-1 resulted in organ specific autoimmune disease.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This DTA-1 antibody has been reported for use in flow cytometric analysis. It has also been reported for in vitro and in vivo blocking of mouse GITR. (Please use Functional Grade purified DTA-1 in functional assays.)

Applications Tested

This DTA-1 antibody has been tested by flow cytometric analysis of mouse splenocyte suspension. This can be used at less than or equal to 0.015 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Shimizu J, Yamazaki S, Takahashi T, Ishida Y, Sakaguchi S. Stimulation of CD25(+)CD4(+) regulatory T cells through GITR breaks immunological self-tolerance. Nat Immunol. 2002 Feb;3(2):135-42.

Related Products

Cat. 16-4031 Functional Grade Purified Rat IgG2b Isotype Control